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          • Primary Antibodies ›
          • Carbonic Anhydrase I Antibodies

          Invitrogen

          Carbonic Anhydrase I Recombinant Rabbit Monoclonal Antibody (26E3)

          View all (34) Carbonic Anhydrase I antibodies
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          Datasheet
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          Cite Carbonic Anhydrase I Recombinant Rabbit Monoclonal Antibody (26E3)

          • Antibody Testing Data (3)
          Carbonic Anhydrase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Carbonic Anhydrase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Carbonic Anhydrase I Antibody (MA5-50151) in IHC (P)

          Immunohistochemistry analysis with a Carbonic Anhydrase I monoclonal antibody (Product # MA5-50151) using a dilution of 1:50 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Carbonic Anhydrase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Carbonic Anhydrase I Antibody in Western Blot (WB)
          Carbonic Anhydrase I Antibody in Flow Cytometry (Flow)

          Product Details

          MA5-50151

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:50-1:200
          -

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          26E3

          Immunogen

          A synthesized peptide derived from Human CA1

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.75 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3092531

          Target Information

          Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: CA-I; CAB; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase B; Carbonic anhydrase I; Cyanamide hydratase CA1; epididymis secretory protein Li 11

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          Gene Aliases: CA-I; CA1; CAB; Car1; HEL-S-11

          View more View less

          UniProt ID: (Human) P00915

          View more View less

          Entrez Gene ID: (Human) 759

          View more View less

          Function(s)
          arylesterase activity carbonate dehydratase activity protein binding zinc ion binding hydro-lyase activity
          Process(es)
          one-carbon metabolic process bicarbonate transport
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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