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          • Primary Antibodies ›
          • Carbonic Anhydrase III Antibodies

          Invitrogen

          Carbonic Anhydrase III Recombinant Rabbit Monoclonal Antibody (006)

          View all (31) Carbonic Anhydrase III antibodies

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          Cite Carbonic Anhydrase III Recombinant Rabbit Monoclonal Antibody (006)

          • Antibody Testing Data (2)
          Carbonic Anhydrase III Antibody in Western Blot (WB)
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          Carbonic Anhydrase III Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Carbonic Anhydrase III Antibody (MA5-29072) in WB

          Western Blot using Carbonic Anhydrase III Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29072) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Carbonic Anhydrase III Antibody in Western Blot (WB)
          Carbonic Anhydrase III Antibody in Immunoprecipitation (IP)

          Product Details

          MA5-29072

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunoprecipitation (IP)

          1-4 µL/mg of lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          006

          Immunogen

          Recombinant Human Carbonic Anhydrase III/CA3 protein (Met1-Lys260)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2785002

          Product Specific Information

          This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          This antibody has specificity for Human Carbonic Anhydrase III/CA3.

          Target Information

          Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10. 3 kb and contains seven exons and six introns.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CA III; CA-III; CAH3; CAIII (Muscle); Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1; epididymis secretory sperm binding protein Li 167mP; HEL-S-167mP

          View more View less

          Gene Aliases: CA3; CAIII; Car3

          View more View less

          UniProt ID: (Human) P07451

          View more View less

          Entrez Gene ID: (Human) 761

          View more View less

          Function(s)
          carbonate dehydratase activity zinc ion binding nickel cation binding phosphatase activity
          Process(es)
          one-carbon metabolic process response to oxidative stress bicarbonate transport dephosphorylation response to ethanol
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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