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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Carboxypeptidase Y Antibodies

          Invitrogen

          Carboxypeptidase Y Monoclonal Antibody (10A5B5)

          Advanced Verification
          17 Published Figures
          60 References
          View all (8) Carboxypeptidase Y antibodies

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          Cite Carboxypeptidase Y Monoclonal Antibody (10A5B5)

          • Published Figures (17)
          • Advanced Verification (1)
          Carboxypeptidase Y Antibody in Western Blot (WB)
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          Carboxypeptidase Y Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 18

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          Carboxypeptidase Y Antibody (A-6428) in WB

          Nature communications 2023 - Genipin targets endocytosis and lipid storage to protect yeast from alphaSyn-GFP toxicity. a Endocytosis evaluation by FM4-64 incubation by confocal microscopy. mRNA levels of b YPT1 (E vs A * p = 0.0446; A vs AG * p = 0.0354, n = 4) c HRR25 (E vs G * p = 0.0458; E vs A * p = 0.0307; A vs AG * p = 0.0327, n = 3) and d YPK1 (E vs G *** p = 0.0004; A vs AG p = 0.0039, n = 3). e... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in Western Blot (WB)
          Carboxypeptidase Y Antibody in ELISA (ELISA)
          Carboxypeptidase Y Antibody in ELISA (ELISA)
          Carboxypeptidase Y Antibody in Dot Blot (DB)
          Carboxypeptidase Y Antibody in Dot Blot (DB)
          Carboxypeptidase Y Antibody in Dot Blot (DB)
          Carboxypeptidase Y Antibody

          Product Details

          A-6428

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25 µg/mL
          View 40 publications 40 publications

          Immunocytochemistry (ICC/IF)

          20 µg/mL
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 9 publications 9 publications

          Dot blot (DB)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Yeast

          Published species

          Yeast

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          10A5B5

          Immunogen

          Purified yeast CPY (carboxypeptidase)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Ig fraction

          Storage buffer

          HEPES buffered saline

          Contains

          0.02% sodium azide

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536203

          Product Specific Information

          Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.

          Target Information

          Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; cpy1; pcy1

          View more View less

          Gene Aliases: LBC1; PRC1; YMR297W

          View more View less

          UniProt ID: (Baker's yeast) P00729

          View more View less

          Entrez Gene ID: (Baker's yeast) 855343

          View more View less

          Function(s)
          carboxypeptidase activity serine-type carboxypeptidase activity peptidase activity hydrolase activity
          Process(es)
          proteolysis protein catabolic process in the vacuole phytochelatin biosynthetic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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