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Invitrogen
Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)
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Cardiac Myosin Heavy Chain Antibody (MA1-26180) in WB
Experimental and therapeutic medicine 2021 -
Figure 2 HQQR improves Ang II-induced cardiomyocyte hypertrophy. (A) Immunofluorescence experiments were performed using anti-alpha-actinin antibodies to analyze cardiomyocyte hypertrophy (magnification, x200; scale bar, 50 um). (B) mRNA expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using reverse transcription-quantitative PCR. (C) Protein expression levels of t... View More
Product Details
MA1-26180
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Pig,
Rabbit,
Rat
Published species
Human,
Mouse,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3-48
Immunogen
Full length native protein (purified) (Human).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
IgG fraction
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2147173
Product Specific Information
This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.
Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.
Target Information
Cardiac Myosin Heavy Chain (MHC) is a crucial component of the contractile machinery in heart muscle cells. It primarily forms part of the thick filament in sarcomeres, the fundamental units of muscle contraction. The cardiac myosin heavy chain comes in two isoforms, alpha-MHC and beta-MHC, which are encoded by the MYH6 and MYH7 genes, respectively. The alpha-MHC isoform is predominant in the atria and is characterized by faster ATPase activity and contraction speed, whereas the beta-MHC isoform is predominant in the ventricles and is associated with greater endurance. Each myosin molecule consists of two heavy chains, two essential light chains (ELC), and two regulatory light chains (RLC). The heavy chains have distinct functional regions: the globular head, which binds to actin and hydrolyzes ATP to produce force, and the helical tail, which organizes into the thick filament backbone. Mutations in cardiac MHC can lead to various cardiomyopathies, including hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), both of which significantly affect the heart’s functionality. These disorders underscore the importance of MHC in maintaining normal cardiac physiology and highlight its complex regulation and mechanotransduction capabilities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha cardiac MHC; alpha myosin; alpha-cardiac myosin heavy chain; Antigen MLAA-21; beta cardiac myosin heavy chain; beta myosin heavy chain; beta-myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain alpha isoform; cardiac myosin heavy chain beta isoform; cardiomyopathy, hypertrophic 1; MYH-beta/slow; MyHC-alpha; MyHC-beta; MyHC-slow; myopathy, distal 1; myosin 7; Myosin cardiac muscle beta chain; Myosin heavy chain 6; Myosin heavy chain 7; Myosin heavy chain 7B, cardiac muscle beta isoform; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain polypeptide 7 cardiac muscle fetal; Myosin heavy chain slow isoform; myosin heavy chain slow type 1 (beta cardiac); Myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); Myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, adult; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 6, cardiac muscle, alpha; myosin heavy chain, polypeptide 7; myosin, heavy chain 6, cardiac muscle, alpha; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy chain 7B, cardiac muscle, beta; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy polypeptide 7B, cardiac muscle, beta; Myosin-6; Myosin-7; Myosin-7B; rhabdomyosarcoma antigen MU-RMS-40.7A; Slow A MYH14; type slow/beta myosin heavy chain; U937-associated antigen
Gene Aliases: A830009F23Rik; AA517445; alpha-MHC; ASD3; B-MHC; beta-MHC; BOS_10147; CMD1EE; CMD1S; CMH1; CMH14; KIAA1512; MHC14; MPD1; MYH14; MYH6; MYH7; MYH7B; MYHC; Myhc-a; Myhc-b; MyHC-I; MYHCA; MYHCB; SPMD; SPMM; SSS3
microfilament motor activity
actin binding
calmodulin binding
ATP binding
ATPase activity
myosin phosphatase activity
protein kinase binding
actin-dependent ATPase activity
motor activity
protein binding
protein homodimerization activity
protein heterodimerization activity
nucleotide binding
calcium-dependent ATPase activity
identical protein binding
in utero embryonic development
regulation of the force of heart contraction
regulation of heart rate
muscle contraction
striated muscle contraction
adult heart development
visceral muscle development
regulation of heart contraction
metabolic process
regulation of blood pressure
actin filament-based movement
muscle filament sliding
myofibril assembly
BMP signaling pathway
regulation of ATPase activity
sarcomere organization
ATP metabolic process
cardiac muscle fiber development
atrial cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
canonical Wnt signaling pathway
regulation of heart growth
response to heat
response to reactive oxygen species
transition between fast and slow fiber
skeletal muscle contraction
regulation of the force of skeletal muscle contraction
regulation of slow-twitch skeletal muscle fiber contraction
protein dephosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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