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Cardiac Troponin I Monoclonal Antibody (1D5D6)

Cardiac Troponin I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cardiac Troponin I Antibody (66376-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cardiac Troponin I Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Cardiac Troponin I Antibody in Western Blot (WB)

Product Image Gallery

Product Details

66376-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D5D6

Immunogen

Cardiac Troponin I Fusion Protein Ag16758 (1-110 aa encoded by BC096165)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to cTnI, but not cross-react with skeletal TnI.

Immunogen sequence: MADGSSDAA REPRPAPAPI RRRSSNYRAY ATEPHAKKKS KISASRKLQL KTLLLQIAKQ ELEREAEERR GEKGRALSTR CQPLELAGLG FAELQDLCRQ LHARVDKVDE E (1-110 aa encoded by BC096165)

Target Information

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic β-adrenergic stress contributes to cardiomyopathy in rodents with collagen-induced arthritis.

Acta pharmacologica Sinica

Zhu ZD,Zhang M,Wang Z,Jiang CR,Huang CJ,Cheng HJ,Guan QY,Su TT,Wang MM,Gao Y,Wu HF,Wei W,Han YS,Wang QT

Sun Oct 01 00:00:00 UTC 2023

Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection.

Stem cell research & therapy

Huang A,Liu Y,Qi X,Chen S,Huang H,Zhang J,Han Z,Han ZC,Li Z

Fri Jun 17 00:00:00 UTC 2022

CUGBP1, a crucial factor for heart regeneration in mice.

Cell death & disease

Liu Y,Wang H,Zhang H,Wang J,Liu Q,Bi Y,Song S,Qiao X,Zhu K,Wu Y,Ji G

Tue Feb 08 00:00:00 UTC 2022

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue.

Stem cell reports

Shen S,Sewanan LR,Shao S,Halder SS,Stankey P,Li X,Campbell SG

Tue Sep 13 00:00:00 UTC 2022

Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.

Frontiers in pharmacology

Zheng H,Xu N,Zhang Z,Wang F,Xiao J,Ji X

Sat Jul 16 00:00:00 UTC 2022

A handheld testing device for the fast and ultrasensitive recognition of cardiac troponin I via an ion-sensitive field-effect transistor.

Biosensors & bioelectronics

Wang Y,Liu T,Yang M,Wu C,Zhang W,Chu Z,Jin W

Wed Dec 01 00:00:00 UTC 2021

DBA/1 mice display equivalent cardiac function to C57BL/6J mice.

Experimental physiology

Wang Z,Zhou Z,Guo P,Wang M,Sun H,Tai Y,Xiao F,Han Y,Wei W,Wang Q

Thu Apr 01 00:00:00 UTC 2021

Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress.

Journal of cellular and molecular medicine

Lyu L,Chen J,Wang W,Yan T,Lin J,Gao H,Li H,Lv R,Xu F,Fang L,Chen Y

Mon Mar 01 00:00:00 UTC 2021

Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.

Journal of the American Heart Association

Thirugnanam K,Cossette SM,Lu Q,Chowdhury SR,Harmann LM,Gupta A,Spearman AD,Sonin DL,Bordas M,Kumar SN,Pan AY,Simpson PM,Strande JL,Bishop E,Zou MH,Ramchandran R

Tue Nov 19 00:00:00 UTC 2019

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Experimental and therapeutic medicine

Ding C,Yang C,Cao Q,Zhu X,Zhang J,Zhang W,Wang Y,Li L

Fri Sep 01 00:00:00 UTC 2017

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Experimental and therapeutic medicine

Ding C,Yang C,Cao Q,Zhu X,Zhang J,Zhang W,Wang Y,Li L

Fri Sep 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); MGC116817; Tpn1; troponin 1, type 3; troponin I type 3 (cardiac); Troponin I, cardiac muscle

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Gene Aliases: CMD1FF; CMD2A; CMH7; Ctni; RCM1; Tn1; TnI; TNNC1; TNNI3

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UniProt ID: (Human) P19429, (Rat) P23693, (Mouse) P48787

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Entrez Gene ID: (Human) 7137, (Rat) 29248, (Mouse) 21954

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Function(s)

actin binding protein binding calcium channel inhibitor activity protein kinase binding protein domain specific binding troponin C binding troponin T binding metal ion binding calcium-dependent protein binding

Process(es)

vasculogenesis regulation of systemic arterial blood pressure by ischemic conditions cellular calcium ion homeostasis regulation of muscle contraction regulation of smooth muscle contraction striated muscle contraction heart development negative regulation of ATPase activity ventricular cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction skeletal muscle contraction muscle filament sliding regulation of cardiac conduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cardiac Troponin I Monoclonal Antibody (1D5D6)

Cardiac Troponin I Antibody (66376-1-IG) in IHC (P)

Product Details

66376-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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