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Invitrogen
Cas12j Polyclonal Antibody
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FIGURE: 1 / 2
Cas12j Antibody (PA5-143597) in ICC/IF
Immunofluorescent analysis of Cas12j in HEK293 cells. Samples were stained with Cas12j polyclonal antibody (Product # PA5-143597) in red. Cells were transfected with pCI-Neo-Mod vector including DNA encoding full length Cas theta protein and stained with rabbit pAb against Cas theta (Product # PA5-143597) in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the Cas theta protein only in transfected cells.
Product Details
PA5-143597
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length Cas12j expressed in and purified from E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2942826
Target Information
There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. The lab of Nobel prize winner Jennifer Doudna characterized these Cas enzymes and showed that they could be used to perform CRISPR, but had the significant advantage that they were much smaller in molecular size than other Cas family enzymes such as the widely used Cas9 enzymes from S. pyogenes and S. aureus. One of these enzymes is referred to as Cas theta and also known as Cas-12j. The use of such smaller Cas family enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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