Invitrogen

Caspase 1 Monoclonal Antibody (14F468)

Caspase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Caspase 1 Antibody (MA5-16215) in IHC (P)

Immunohistochemical analysis of Caspase 1 in Normal lung from human. Samples were incubated in Caspase 1 monoclonal antibody (Product # MA5-16215) using a dilution of 5 µg/mL. In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnif... View More

Caspase 1 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-16215

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

14F468

Immunogen

Two synthetic peptides from the human Caspase-1 protein (amino acids 371-390 and 31-45).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537733

Product Specific Information

Suggested positive control: 3T3 whole cell lysate, antigen standard for CASP1 (transient overexpression lysate), HeLa or NIH-3T3, Hela whole cell lysate.

Target Information

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis.

Pharmaceuticals (Basel, Switzerland)

Salem HA,Elsherbiny N,Alzahrani S,Alshareef HM,Abd Elmageed ZY,Ajwah SM,Hamdan AME,Abdou YS,Galal OO,El Azazy MKA,Abu-Elfotuh K

MA5-16215 was used in Western Blotting to explore the association between monosodium glutamate (MSG) , protein malnutrition, and induced brain injury similar to attention-deficit/hyperactivity disorder (ADHD).

Wed Aug 17 00:00:00 UTC 2022

Gasdermin D: in vivo evidence of pyroptosis in spontaneous labor at term.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

Gomez-Lopez N,Romero R,Panaitescu B,Miller D,Zou C,Gudicha DW,Tarca AL,Para R,Pacora P,Hassan SS,Hsu CD

MA5-16215 was used in Immunohistochemistry (Paraffin) to provide evidence implicating pyroptosis in the mechanisms that lead to the sterile inflammatory process of term parturition.

Mon Feb 01 00:00:00 UTC 2021

Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection.

American journal of reproductive immunology (New York, N.Y. : 1989)

Gomez-Lopez N,Romero R,Tarca AL,Miller D,Panaitescu B,Schwenkel G,Gudicha DW,Hassan SS,Pacora P,Jung E,Hsu CD

MA5-16215 was used in Immunohistochemistry to show that pyroptosis can occur in the context of sterile intra-amniotic inflammation and intra-amniotic infection in patients with spontaneous preterm labor and birth.

Sun Dec 01 00:00:00 UTC 2019

Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection.

American journal of reproductive immunology (New York, N.Y. : 1989)

Gomez-Lopez N,Romero R,Tarca AL,Miller D,Panaitescu B,Schwenkel G,Gudicha DW,Hassan SS,Pacora P,Jung E,Hsu CD

Sun Dec 01 00:00:00 UTC 2019

Inflammasome activation during spontaneous preterm labor with intra-amniotic infection or sterile intra-amniotic inflammation.

American journal of reproductive immunology (New York, N.Y. : 1989)

Gomez-Lopez N,Romero R,Panaitescu B,Leng Y,Xu Y,Tarca AL,Faro J,Pacora P,Hassan SS,Hsu CD

MA5-16215 was used in Immunohistochemistry to investigate inflammasome activation in the context of intra-amniotic infection (IAI) or sterile intra-amniotic inflammation (SIAI). Extracellular ASC concentrations, indicative of inflammasome activation, were measured by ELISA, and the expression of ASC, caspase-1, and interleukin (IL)-1β was assessed in chorioamniotic membranes using multiplex immunofluorescence.

Thu Nov 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466, (Mouse) P29452

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Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166

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Function(s)

endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic process peptidase activity cysteine-type peptidase activity hydrolase activity scaffold protein binding

Process(es)

response to hypoxia proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction regulation of autophagy protein processing response to lipopolysaccharide interleukin-1 beta production response to ATP regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion regulation of inflammatory response mitochondrial depolarization membrane hyperpolarization pyroptosis cellular response to mechanical stimulus cellular response to organic substance programmed necrotic cell death toxin transport microglial cell activation myoblast fusion memory response to bacterium response to organic cyclic compound response to drug positive regulation of apoptotic process positive regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of cytokine secretion midgut development response to toxic substance lung development execution phase of apoptosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 1 Monoclonal Antibody (14F468)

Caspase 1 Antibody (MA5-16215) in IHC (P)

Product Details

MA5-16215

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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