Invitrogen

Caspase 1 Monoclonal Antibody (5B10), eBioscience™

FIGURE: 1 / 14

Caspase 1 Antibody (14-9832-82) in WB

Lysates prepared from the Jurkat (lane 1) and J774 (lane 2) cell lines under reducing conditions with DTT were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Mouse Caspase 1 Purified. Bands were visualized using Anti-Rat IgG HRP.

Caspase 1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

14-9832-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2016691

Product Specific Information

Description: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis. In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme). Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits. There are several isoforms of Caspase-1.

The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form.

Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Significance of Plasmodium berghei Amino Acid Transporter 1 in Food Vacuole Functionality and Its Association with Cerebral Pathogenesis.

Microbiology spectrum

Anand A,Chandana M,Ghosh S,Das R,Singh N,Vaishalli PM,Gantasala NP,Padmanaban G,Nagaraj VA

14-9832-82 was used in Western Blotting to show that the deletion of amino acid transporter 1 in Plasmodium berghei leads to swollen food vacuoles with the accumulation of hemoglobin-derived peptides.

Tue Mar 28 00:00:00 UTC 2023

The p-STAT3/ANXA2 axis promotes caspase-1-mediated hepatocyte pyroptosis in non-alcoholic steatohepatitis.

Journal of translational medicine

Feng Y,Li W,Wang Z,Zhang R,Li Y,Zang L,Wang P,Li Z,Dong Y

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Wed Nov 02 00:00:00 UTC 2022

Glycosylation-Dependent Induction of Programmed Cell Death in Murine Adenocarcinoma Cells.

Frontiers in immunology

Parshenkov A,Hennet T

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Fri Apr 08 00:00:00 UTC 2022

Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.

Nature communications

Chandana M,Anand A,Ghosh S,Das R,Beura S,Jena S,Suryawanshi AR,Padmanaban G,Nagaraj VA

14-9832-82 was used in Western Blotting to show heme synthesized in asexual stages promotes cerebral pathogenesis by enhancing hemozoin formation.

Tue Jul 12 00:00:00 UTC 2022

Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

Nature cardiovascular research

Mantri M,Hinchman MM,McKellar DW,Wang MFZ,Cross ST,Parker JSL,De Vlaminck I

14-9832-82 was used in Immunohistochemistry to show a complex network of cellular phenotypes and spatially restricted cell-cell interactions associated with reovirus-induced myocarditis in neonatal mice.

Sat Oct 01 00:00:00 UTC 2022

Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome.

Nature immunology

Moretti J,Jia B,Hutchins Z,Roy S,Yip H,Wu J,Shan M,Jaffrey SR,Coers J,Blander JM

14-9832-82 was used in Western Blotting to find that bacterial mRNA, which signals bacterial viability, was required alongside LPS for noncanonical activation of the NLRP3 inflammasome in macrophages.

Sun May 01 00:00:00 UTC 2022

Adipocyte CD1d Gene Transfer Induces T Cell Expansion and Adipocyte Inflammation in CD1d Knockout Mice.

Journal of immunology (Baltimore, Md. : 1950)

Xiao R,Mansour AG,Huang W,Hassan QN,Wilkins RK,Komatineni SV,Bates R,Ali S,Chrislip LA,Queen NJ,Ma S,Yu J,Lordo MR,Mundy-Bosse BL,Caligiuri MA,Cao L

14-9832-82 was used in Western Blotting to report that adeno-associated virus-mediated gene transfer of CD1d to adipocytes in CD1d KO mice fails to rescue iNKT cells but leads to massive and selective expansion of T cells within adipose tissue, particularly CD8+ T effector cells, that is associated with adipocyte NLRP3 inflammasome activation, dysregulation of adipocyte functional genes, and upregulation of apoptotic pathway proteins.

Sun May 01 00:00:00 UTC 2022

Modulation of the NLRP3 inflammasome by Sars-CoV-2 Envelope protein.

Scientific reports

Yalcinkaya M,Liu W,Islam MN,Kotini AG,Gusarova GA,Fidler TP,Papapetrou EP,Bhattacharya J,Wang N,Tall AR

Published figure using Caspase 1 monoclonal antibody (Product # 14-9832-82) in Western Blot

Fri Dec 24 00:00:00 UTC 2021

Integrative metabolomics and transcriptomics identifies itaconate as an adjunct therapy to treat ocular bacterial infection.

Cell reports. Medicine

Singh S,Singh PK,Jha A,Naik P,Joseph J,Giri S,Kumar A

14-9832-82 was used in Western Blot to study the anti-inflammatory effects of itaconate using integrated metabolomics and transcriptomics.

Tue May 18 00:00:00 UTC 2021

NLPR3 inflammasome inhibition alleviates hypoxic endothelial cell death in vitro and protects blood-brain barrier integrity in murine stroke.

Cell death & disease

Bellut M,Papp L,Bieber M,Kraft P,Stoll G,Schuhmann MK

14-9832-82 was used in Immunohistochemistry (Frozen) to study the survival of brain endothelial cells exposed to oxygen and glucose deprivation in vitro in the context of inflammasome inhibition with the NLR-family pyrin domain-containing protein 3 (NLRP3)-specific drug MCC950.

Mon Dec 20 00:00:00 UTC 2021

Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis.

Immunity

Tummers B,Mari L,Guy CS,Heckmann BL,Rodriguez DA,Rühl S,Moretti J,Crawford JC,Fitzgerald P,Kanneganti TD,Janke LJ,Pelletier S,Blander JM,Green DR

14-9832-82 was used in Western Blot to suggest an in vivo role of the inflammatory RIPK1-caspase-8-FADD (FADDosome) complex and reveal a FADD-independent inflammatory role of caspase-8 that involves activation of an inflammasome.

Tue Jun 16 00:00:00 UTC 2020

An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.

Cell metabolism

He M,Chiang HH,Luo H,Zheng Z,Qiao Q,Wang L,Tan M,Ohkubo R,Mu WC,Zhao S,Wu H,Chen D

14-9832 was used in Western Blotting to establish the dysregulation of the acetylation switch of the NLRP3 inflammasome as an origin of aging-associated chronic inflammation and highlight the reversibility of aging-associated chronic inflammation and insulin resistance.

Tue Mar 03 00:00:00 UTC 2020

Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting.

Nature communications

Brocker CN,Kim D,Melia T,Karri K,Velenosi TJ,Takahashi S,Aibara D,Bonzo JA,Levi M,Waxman DJ,Gonzalez FJ

14-9832 was used in Western Blotting to show nuclear receptor PPARα reduces inflammasome activation through induction of long non-coding RNA Gm15441 as a result of metabolic stress.

Tue Nov 17 00:00:00 UTC 2020

Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting.

Nature communications

Brocker CN,Kim D,Melia T,Karri K,Velenosi TJ,Takahashi S,Aibara D,Bonzo JA,Levi M,Waxman DJ,Gonzalez FJ

14-9832 was used in Western Blotting to show nuclear receptor PPARα reduces inflammasome activation through induction of long non-coding RNA Gm15441 as a result of metabolic stress.

Tue Nov 17 00:00:00 UTC 2020

Cutting Edge: TAK1 Safeguards Macrophages against Proinflammatory Cell Death.

Journal of immunology (Baltimore, Md. : 1950)

Sanjo H,Nakayama J,Yoshizawa T,Fehling HJ,Akira S,Taki S

14-9832-82 was used in Cell Culture to reveal that TAK1 acts as a signaling checkpoint to protect macrophages from unique proinflammatory cell death, ensuring the maintenance of innate immune homeostasis.

Thu Aug 15 00:00:00 UTC 2019

Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging.

Cell reports

Luo H,Mu WC,Karki R,Chiang HH,Mohrin M,Shin JJ,Ohkubo R,Ito K,Kanneganti TD,Chen D

14-9832 was used in Western Blotting to assess whether the NLRP3 inflammasome is aberrantly activated in haematopoietic stem cells during physiological ageing.

Tue Jan 22 00:00:00 UTC 2019

Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging.

Cell reports

Luo H,Mu WC,Karki R,Chiang HH,Mohrin M,Shin JJ,Ohkubo R,Ito K,Kanneganti TD,Chen D

14-9832 was used in Western Blotting to assess whether the NLRP3 inflammasome is aberrantly activated in haematopoietic stem cells during physiological ageing.

Tue Jan 22 00:00:00 UTC 2019

Intra-amniotic inflammation induces preterm birth by activating the NLRP3 inflammasome†.

Biology of reproduction

Faro J,Romero R,Schwenkel G,Garcia-Flores V,Arenas-Hernandez M,Leng Y,Xu Y,Miller D,Hassan SS,Gomez-Lopez N

14-9832-82 was used in Western Blotting to investigate effects of NLRP3 inflammosome induction on preterm birth.

Wed May 01 00:00:00 UTC 2019

Free immunoglobulin light chain (FLC) promotes murine colitis and colitis-associated colon carcinogenesis by activating the inflammasome.

Scientific reports

Ma J,Jiang D,Gong X,Shao W,Zhu Z,Xu W,Qiu X

14-9832 was used in Western Blotting to investigate whether free Ig light chain is involved in colitis and colitis-associated colon carcinogenesis.

Wed Jul 12 00:00:00 UTC 2017

Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.

Nature medicine

Lood C,Blanco LP,Purmalek MM,Carmona-Rivera C,De Ravin SS,Smith CK,Malech HL,Ledbetter JA,Elkon KB,Kaplan MJ

14-9832 was used in Western Blotting to investigate how neutrophil extracellular traps are generated and their roles in sterile inflammation.

Mon Feb 01 00:00:00 UTC 2016

Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.

The Journal of biological chemistry

Marty-Roix R,Vladimer GI,Pouliot K,Weng D,Buglione-Corbett R,West K,MacMicking JD,Chee JD,Wang S,Lu S,Lien E

14-9832 was used in Western Blotting to determine the mechanisms of cellular action induced by QS-21.

Fri Jan 15 00:00:00 UTC 2016

Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury.

Nature communications

Ito M,Shichita T,Okada M,Komine R,Noguchi Y,Yoshimura A,Morita R

14-9832 was used in Immunoprecipitation to report that Bruton's tyrosine kinase (BTK) is an essential component of the NLRP3 inflammasome, in which BTK physically interacts with ASC and NLRP3.

Wed Jun 10 00:00:00 UTC 2015

Nitric oxide controls the immunopathology of tuberculosis by inhibiting NLRP3 inflammasome-dependent processing of IL-1β.

Nature immunology

Mishra BB,Rathinam VA,Martens GW,Martinot AJ,Kornfeld H,Fitzgerald KA,Sassetti CM

14-9832 was used in Western Blotting to investigate the mechanisms that controls IL-1 during tuberculosis.

Tue Jan 01 00:00:00 UTC 2013

Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation.

Journal of immunology (Baltimore, Md. : 1950)

Kaminski JJ,Schattgen SA,Tzeng TC,Bode C,Klinman DM,Fitzgerald KA

14-9832 was used in Western Blotting to investigate the effect of ODN-A151 in dendritic cells and macrophages.

Tue Oct 01 00:00:00 UTC 2013

TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria.

Cell

Rathinam VA,Vanaja SK,Waggoner L,Sokolovska A,Becker C,Stuart LM,Leong JM,Fitzgerald KA

14-9832 was used in Western Blotting to investigate the molecular mechanisms underlying "sepsis syndrome" response during systemic Gram-negative bacteria infection.

Fri Aug 03 00:00:00 UTC 2012

Chitosan but not chitin activates the inflammasome by a mechanism dependent upon phagocytosis.

The Journal of biological chemistry

Bueter CL,Lee CK,Rathinam VAK,Healy GJ,Taron CH,Specht CA,Levitz SM

14-9832 was used in Western Blotting to investigate the capacity of chitin to stimulate murine macrophages to release the inflammasome-associated cytokine IL-1β.

Fri Oct 14 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CASP-1; Caspase-1; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; interleukin 1 beta-converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

View more View less

Gene Aliases: Casp1; ICE; Il1bc

View more View less

UniProt ID: (Mouse) P29452

View more View less

Entrez Gene ID: (Mouse) 12362

View more View less

Function(s)

endopeptidase activity cysteine-type endopeptidase activity protein binding peptidase activity cysteine-type peptidase activity hydrolase activity scaffold protein binding

Process(es)

response to hypoxia microglial cell activation proteolysis apoptotic process myoblast fusion memory response to bacterium regulation of autophagy response to organic cyclic compound protein processing response to lipopolysaccharide interleukin-1 beta production response to ATP response to drug regulation of apoptotic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion regulation of inflammatory response mitochondrial depolarization membrane hyperpolarization pyroptosis cellular response to organic substance programmed necrotic cell death toxin transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Caspase 1 Monoclonal Antibody (5B10), eBioscience™

Caspase 1 Antibody (14-9832-82) in WB

Product Details

14-9832-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!