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Invitrogen
Caspase 11 Monoclonal Antibody (17D9), eBioscience™
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FIGURE: 1 / 5
Caspase 11 Antibody (14-9935-82) in WB
Lysates prepared under reducing conditions from J774 cells (lane 1) or LPS stimulated mouse splenocytes (lane 2) or unstimulated splenocytes (lanes 3) were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Mouse Caspase 11 Purified.Bands were visualized using Anti-Rat IgG HRP.
Product Details
14-9935-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
17D9
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1518790
Product Specific Information
Description: The monoclonal antibody 17D9 recognizes mouse Caspase-11, a cysteine protease that plays a role in apoptosis and inflammation. Caspase-11 exists as a 43 kDa and 38 kDa pro-caspase form and a 30 and 10 kDa active form. The 17D9 antibody recognizes p43, p38 and p30 forms. Caspase-11 is an upstream protease that activates other caspases, such as caspase-3, -7 and -1, which in turn activate IL-1beta. Under Caspase-11-deficient conditions, cells are unable to process or secrete IL-1beta in response to stimuli and show defects in motility, which are due to disruption of its interaction with Aip1, an actin binding protein. Caspase-11 is homologous to human Caspase-4 (around 60%). Unlike other caspases which tend to be constitutively expressed, caspase-11 is upregulated by LPS treatment in macrophages, lymphocytes and hepatocytes.
Applications Reported: This 17D9 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 17D9 antibody has been tested by western blotting at 1-5 µg/mL on J774 cells or stimulated splenocyte lysates. Samples must be reduced for detection. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CASP-11; CASP-4; Caspase-11; Caspase-4; Protease ICH-3
Gene Aliases: CASP-11; CASP-4; Casp11; Casp4; Caspl; ich-3; Ich3
UniProt ID: (Mouse) P70343
Entrez Gene ID: (Mouse) 12363
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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