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Caspase 12 Antibody (600-401-MJ3) in ICC/IF
Immunofluorescence of Rabbit Anti-Caspase 12 Antibody. Cells: NIH/3T3 Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Caspase 12 Antibody at 15µg/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 15µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Caspase 12 + Dylight™488. (C) Merge A+B.... View More
Product Details
600-401-MJ3
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Caspase 12 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Caspase 12 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Caspase 12. A BLAST analysis was used to suggest reactivity with the antigen based on 100% homology with the immunizing sequence.
Target Information
Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including -gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP-12; Caspase-12; OTTMUSP00000045799; OTTMUSP00000045802; OTTMUSP00000045803; UNQ9415
Gene Aliases: Casp12
UniProt ID: (Rat) Q920D5, (Mouse) O08736
Entrez Gene ID: (Rat) 156117, (Mouse) 12364
proteolysis
apoptotic process
aging
response to cold
response to inorganic substance
response to iron(III) ion
response to organonitrogen compound
protein autoprocessing
endoplasmic reticulum unfolded protein response
response to anoxia
response to endoplasmic reticulum stress
response to nicotine
response to drug
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to ethanol
regulation of inflammatory response
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
execution phase of apoptosis
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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