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Invitrogen
Caspase 2 Precursor Recombinant Rabbit Monoclonal Antibody (2Z10F1)
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Caspase 2 Precursor Antibody (MA5-35599) in WB
Western blot analysis of Caspase 2 Precursor in extracts of various cell lines. Samples were incubated with Caspase 2 Precursor Monoclonal antibody (Product # MA5-35599) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Staurosporine (1 µM) for 3 hours. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-35599
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2Z10F1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 334-452 of human Caspase-2 (P29594)
Conjugate
Form
Liquid
Concentration
2.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849499
Product Specific Information
Immunogen sequence: GKNHTQSPGC EESDAGKEEL MKMRLPTRSD MICGYACLKG NAAMRNTKRG SWYIEALTQV FSERACDMHV ADMLVKVNAL IKEREGYAPG TEFHRCKEMS EYCSTLCQQL YLFPGYPPT
Target Information
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP-2; caspase 2 apoptosis-related cysteine peptidase; Caspase-2; ICH-1L; ICH-1L/1S; NEDD-2; Neural precursor cell expressed developmentally down-regulated protein 2; Protease ICH-1; protein phosphatase 1, regulatory subunit 57
Gene Aliases: CASP-2; CASP2; ICH1; NEDD-2; NEDD2; PPP1R57
UniProt ID: (Human) P42575
Entrez Gene ID: (Human) 835
luteolysis
neural retina development
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
brain development
aging
intrinsic apoptotic signaling pathway in response to DNA damage
protein processing
ectopic germ cell programmed cell death
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cellular response to mechanical stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
execution phase of apoptosis
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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