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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Caspase 3 Antibodies

          Proteintech

          Caspase 3 Polyclonal Antibody

          1,943 References
          View all (55) Caspase 3 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Caspase 3 Polyclonal Antibody

          • Antibody Testing Data (20)
          Caspase 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Caspase 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 20

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          Caspase 3 Antibody (19677-1-AP) in ICC/IF

          Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Caspase 3 Antibody in Immunocytochemistry (ICC/IF)
          Caspase 3 Antibody in Immunocytochemistry (ICC/IF)
          Caspase 3 Antibody in Immunocytochemistry (ICC/IF)
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Caspase 3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          Caspase 3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          Caspase 3 Antibody in Western Blot (WB)
          Caspase 3 Antibody in Western Blot (WB)
          Caspase 3 Antibody in Western Blot (WB)
          Caspase 3 Antibody in Western Blot (WB)
          Caspase 3 Antibody in Western Blot (WB)
          Caspase 3 Antibody in Flow Cytometry (Flow)
          Caspase 3 Antibody in Immunoprecipitation (IP)

          Product Details

          19677-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 1,657 publications 1,657 publications

          Immunohistochemistry (IHC)

          -
          View 163 publications 163 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 77 publications 77 publications

          Flow Cytometry (Flow)

          Assay-dependent
          -

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 1 publication 1 publication

          RNA Immunoprecipitation (RIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 14 publications 14 publications

          Not applicable (N/A)

          -
          View 29 publications 29 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Hamster, Human, Mouse, Pig, Plant, Rat, Sheep, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody can recognize p17, p19 and p32 of Caspase 3.

          Target Information

          This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

          View more View less

          Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

          View more View less

          UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

          View more View less

          Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

          View more View less

          Function(s)
          protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity
          Process(es)
          response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation
          It has to be done as per old AB suggested Products section.
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