Invitrogen

Caspase 3 Recombinant Rabbit Monoclonal Antibody (9H19L2)

Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Caspase 3 Antibody (700182) in ICC/IF

Immunofluorescence analysis of Caspase-3 was done on 70% confluent log phase HeLa cells treated with 5 uM of Staurosporine for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Caspase-3 (9H19L2) Recombinant Rabbit Monoclonal Antibody (Product # 7001... View More

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Caspase 3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

700182

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H19L2

Immunogen

A peptide corresponding to amino acids 171-175 of P42574.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532293

Product Specific Information

This antibody is predicted to react with bovine, canine, feline, hamster, mouse, primate, pufferfish, rabbit, rat, porcine and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fiber density and matrix stiffness modulate distinct cell migration modes in a 3D stroma mimetic composite hydrogel.

Acta biomaterialia

Hiraki HL,Matera DL,Wang WY,Prabhu ES,Zhang Z,Midekssa F,Argento AE,Buschhaus JM,Humphries BA,Luker GD,Pena-Francesch A,Baker BM

700182 was used in Immunocytochemistry-immunofluorescence to establish a composite approach to modulate fibrous topography and bulk hydrogel mechanics and identified biomaterial parameters to direct distinct 3D cell migration phenotypes.

Thu Jun 01 00:00:00 UTC 2023

Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress.

Antioxidants (Basel, Switzerland)

Wang Z,Li Z,Lei Y,Liu Y,Feng Y,Chen D,Ma S,Xiao Z,Hu M,Deng J,Wang Y,Zhang Q,Huang Y,Yang Y

Published figure using Caspase 3 recombinant monoclonal antibody (Product # 700182) in Western Blot

Tue Nov 22 00:00:00 UTC 2022

FACT maintains nucleosomes during transcription and stem cell viability in adult mice.

EMBO reports

Goswami I,Sandlesh P,Stablewski A,Toshkov I,Safina AF,Magnitov M,Wang J,Gurova K

700182 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that FACT maintains chromatin integrity during transcription in mammalian adult stem cells, suggesting that chromatin transcription in stem cells and differentiated cells is different.

Tue Apr 05 00:00:00 UTC 2022

miR-431-5p regulates apoptosis of cardiomyocytes after acute myocardial infarction via targeting selenoprotein T.

Physiological research

Geng H,Chen L,Su Y,Xu Q,Fan M,Huang R,Li X,Lu X,Pan M

700182 was used in Western Blotting to show that the expression of miR-431-5p in AMI myocardial tissues and H/R-induced HL-1 cardiomyocytes was significantly increased.

Fri Mar 25 00:00:00 UTC 2022

Denatonium as a Bitter Taste Receptor Agonist Modifies Transcriptomic Profile and Functions of Acute Myeloid Leukemia Cells.

Frontiers in oncology

Salvestrini V,Ciciarello M,Pensato V,Simonetti G,Laginestra MA,Bruno S,Pazzaglia M,De Marchi E,Forte D,Orecchioni S,Martinelli G,Bertolini F,Méndez-Ferrer S,Adinolfi E,Di Virgilio F,Cavo M,Curti A

700182 was used in Immunocytochemistry to show that AML cells express functional TAS2Rs.

Fri Aug 09 00:00:00 UTC 2024

HERC1 Ubiquitin Ligase Is Required for Hippocampal Learning and Memory.

Frontiers in neuroanatomy

Pérez-Villegas EM,Pérez-Rodríguez M,Negrete-Díaz JV,Ruiz R,Rosa JL,de Toledo GA,Rodríguez-Moreno A,Armengol JA

700182 was used in Immunohistochemistry to study the tbl hippocampus using behavioral tests, ex vivo electrophysiological recordings, immunohistochemistry, the Golgi-Cox method and transmission electron microscopy.

Fri Nov 10 00:00:00 UTC 2023

HERC1 Ubiquitin Ligase Is Required for Hippocampal Learning and Memory.

Frontiers in neuroanatomy

Pérez-Villegas EM,Pérez-Rodríguez M,Negrete-Díaz JV,Ruiz R,Rosa JL,de Toledo GA,Rodríguez-Moreno A,Armengol JA

Fri Nov 10 00:00:00 UTC 2023

Silencing of soluble epoxide hydrolase 2 gene reduces H₂O₂-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.

Cytotechnology

Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

700182 was used in Western Blotting to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells.

Sat Feb 01 00:00:00 UTC 2020

Repurposing Quinacrine for Treatment of Malignant Mesothelioma: In-Vitro Therapeutic and Mechanistic Evaluation.

International journal of molecular sciences

Kulkarni NS,Vaidya B,Parvathaneni V,Bhanja D,Gupta V

700182 was used in Western Blotting to highlight anti-mesothelioma efficacy of QA at low doses, which can be instrumental in developing it as a stand-alone treatment strategy for MPM.

Mon Aug 31 00:00:00 UTC 2020

Repurposing Quinacrine for Treatment of Malignant Mesothelioma: In-Vitro Therapeutic and Mechanistic Evaluation.

International journal of molecular sciences

Kulkarni NS,Vaidya B,Parvathaneni V,Bhanja D,Gupta V

700182 was used in Western Blotting to highlight anti-mesothelioma efficacy of QA at low doses, which can be instrumental in developing it as a stand-alone treatment strategy for MPM.

Mon Aug 31 00:00:00 UTC 2020

Mitochondrial complex I inhibitor deguelin induces metabolic reprogramming and sensitizes vemurafenib-resistant BRAF^V600E mutation bearing metastatic melanoma cells.

Molecular carcinogenesis

Carpenter EL,Chagani S,Nelson D,Cassidy PB,Laws M,Ganguli-Indra G,Indra AK

700182 was used in Western Blotting to show that treatment with metabolic regulators, can lead to energy starvation and so modulate the intracellular metabolic environment and reducing survival of drug-resistant melanomas harbouring BRAF V600E mutations.

Sun Sep 01 00:00:00 UTC 2019

Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.

International journal of ophthalmology

Che ST,Bie L,Li X,Qi H,Yu P,Zuo L

700182 was used in Western Blotting to deduce that human uveal melanoma cell proliferation can be inhibited and apoptosis induced by parthenolide through the arrest of G1 phase and regulation of the mitochondrial pathway.

Thu Oct 01 00:00:00 UTC 2020

Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.

International journal of ophthalmology

Qian YY,Wu HY,Liu GQ,Ren C,Lu PR,Zhang XG

700182 was used in Western Blotting to investigate the effects of blockade of insulin receptor substrate-1 (IRS-1) on the bio-function of tube formation of human choroidal endothelial cells (HCECs).

Wed Sep 30 00:00:00 UTC 2020

Design of Novel Inhibitors of Human Thymidine Phosphorylase: Synthesis, Enzyme Inhibition, in Vitro Toxicity, and Impact on Human Glioblastoma Cancer.

Journal of medicinal chemistry

de Moura Sperotto ND,Deves Roth C,Rodrigues-Junior VS,Ev Neves C,Reisdorfer Paula F,da Silva Dadda A,Bergo P,Freitas de Freitas T,Souza Macchi F,Moura S,Duarte de Souza AP,Campos MM,Valim Bizarro C,Santos DS,Basso LA,Machado P

700182 was used in Immunohistochemistry to evaluate novel pyrimidine-2,4-diones as inhibitors of human thymidine phosphorylase (hTP) activity.

Thu Feb 14 00:00:00 UTC 2019

Fluid shear stress stimulates breast cancer cells to display invasive and chemoresistant phenotypes while upregulating PLAU in a 3D bioreactor.

Biotechnology and bioengineering

Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

700182 was used in Immunocytochemistry to indicate that pulsatile shear stress promotes breast cancer cell proliferation, invasive potential, chemoresistance, and PLAU signaling.

Fri Nov 01 00:00:00 UTC 2019

1,2,3,4,6-Penta-O-Galloyl-Beta-D-Glucopyranoside Inhibits Proliferation of Multiple Myeloma Cells Accompanied with Suppression of MYC Expression.

Frontiers in pharmacology

Tseeleesuren D,Kant R,Yen CH,Hsiao HH,Chen YA

700182 was used in Western Blotting to provide useful insights into the combination of MYC and proteasome-inhibitors for MM therapy.

Wed Sep 30 00:00:00 UTC 2020

Effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide.

Molecular medicine reports

Shan T,Shan T,Liu F,Zheng H,Li G

700182 was used in Western Blotting to study the effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide.

Wed Feb 01 00:00:00 UTC 2017

Expression of FADD and cFLIP_L balances mitochondrial integrity and redox signaling to substantiate apoptotic cell death.

Molecular and cellular biochemistry

Ranjan K,Pathak C

700182 was used in western blot to elucidate how FADD contributes to mitochondrial-associated apoptosis

Tue Nov 01 00:00:00 UTC 2016

Comparison of 2, 5, and 20 % O2 on the development of post-thaw human embryos.

Journal of assisted reproduction and genetics

Yang Y,Xu Y,Ding C,Khoudja RY,Lin M,Awonuga AO,Dai J,Puscheck EE,Rappolee DA,Zhou C

700182 was used in immunocytochemistry to characterize the development of post-thaw human embryos by comparing 2,5,a nd 20% O2 levels

Fri Jul 01 00:00:00 UTC 2016

Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injury.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Abdel-Hamid AA,Firgany Ael-D,Ali EM

700182 was used in immunohistochemistry - paraffin section to study ethambutol-induced retinal injury and the effect of memantine, an NMDA receptor blocker

Tue Mar 01 00:00:00 UTC 2016

PARP-1 depletion in combination with carbon ion exposure significantly reduces MMPs activity and overall increases TIMPs expression in cultured HeLa cells.

Radiation oncology (London, England)

Ghorai A,Sarma A,Chowdhury P,Ghosh U

700182 was used in immunocytochemistry assess the effects of combining PARP-1 inhibition with carbon ion exposure to control metastatic cells

Thu Sep 22 00:00:00 UTC 2016

Identification of 1,2,3,4,6-Penta-O-galloyl-β-d-glucopyranoside as a Glycine N-Methyltransferase Enhancer by High-Throughput Screening of Natural Products Inhibits Hepatocellular Carcinoma.

International journal of molecular sciences

Kant R,Yen CH,Lu CK,Lin YC,Li JH,Chen YM

700182 was used in western blot to employ the method of high-throughput screening of natural products that inhibit hepatocellular carcinoma by identifying 1,2,3,4,6-Penta-O-galloyl-beta-d-glucopyranoside as a glycine N-methyltransferase enhancer

Wed May 04 00:00:00 UTC 2016

Investigating the role of shape on the biological impact of gold nanoparticles in vitro.

Nanomedicine (London, England)

Tian F,Clift MJ,Casey A,Del Pino P,Pelaz B,Conde J,Byrne HJ,Rothen-Rutishauser B,Estrada G,de la Fuente JM,Stoeger T

700182 was used in western blot to assess the impact of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells.

Tue Jul 19 00:00:00 UTC 2016

Detection and quantification of apoptosis in primary cells using Taqman® protein assay.

Methods in molecular biology (Clifton, N.J.)

Pfister C,Pfrommer H,Tatagiba MS,Roser F

700182 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to describe apoptosis detection and quantification by an innovative method named TaqMan(®) protein assay.

Tue Jun 23 00:00:00 UTC 2015

Cardiac sympathetic afferent denervation attenuates cardiac remodeling and improves cardiovascular dysfunction in rats with heart failure.

Hypertension (Dallas, Tex. : 1979)

Wang HJ,Wang W,Cornish KG,Rozanski GJ,Zucker IH

700182 was used in Immunohistochemistry to suggest a potential new paradigm and therapeutic potential in the management of CHF.

Wed Oct 01 00:00:00 UTC 2014

Impact of diet deprivation and subsequent overallowance during gestation on mammary gland development and lactation performance.

Journal of animal science

Farmer C,Palin MF,Martel-Kennes Y

Wed Jan 01 00:00:00 UTC 2014

Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness.

BMC cancer

Jami MS,Hou J,Liu M,Varney ML,Hassan H,Dong J,Geng L,Wang J,Yu F,Huang X,Peng H,Fu K,Li Y,Singh RK,Ding SJ

700182 was used in immunohistochemistry to study the role of the novel gene KIAA1199 in breast cancer

Sat Mar 15 00:00:00 UTC 2014

Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness.

BMC cancer

Jami MS,Hou J,Liu M,Varney ML,Hassan H,Dong J,Geng L,Wang J,Yu F,Huang X,Peng H,Fu K,Li Y,Singh RK,Ding SJ

700182 was used in immunohistochemistry to study the role of the novel gene KIAA1199 in breast cancer

Sat Mar 15 00:00:00 UTC 2014

Serial diffusion MRI to monitor and model treatment response of the targeted nanotherapy CRLX101.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ng TS,Wert D,Sohi H,Procissi D,Colcher D,Raubitschek AA,Jacobs RE

700182 was used in immunohistochemistry to study the use of serial diffusion MRI to monitor the response to a cyclodextrin polymer nanoparticle containing a DNA topoisomerase I inhibitor in a murine lymphoma model

Wed May 01 00:00:00 UTC 2013

Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay.

Apoptosis : an international journal on programmed cell death

Pfister C,Pfrommer H,Tatagiba MS,Roser F

700182 was used in immunocytochemistry and western blot to develop a TaqMan protein assay of active caspase-3 to quantitate farnesol-induced apoptosis in primary cell cultures

Mon Apr 01 00:00:00 UTC 2013

Apoptosis-dependent acute lung injury and repair after intratracheal instillation of noradrenaline in rats.

Experimental physiology

Uhal BD,Rayford H,Zhuang J,Li X,Laukka J,Soledad-Conrad V

700182 was used in immunohistochemistry - paraffin section to assess intratracheal instillation of noradrenaline in rats and apoptosis-dependent acute lung injury and repair

Sat Mar 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; Cysteine protease CPP32; ICE3; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1

View more View less

Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

View more View less

UniProt ID: (Human) P42574, (Mouse) P70677

View more View less

Entrez Gene ID: (Human) 836, (Mouse) 12367

View more View less

Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Caspase 3 Recombinant Rabbit Monoclonal Antibody (9H19L2)

Caspase 3 Antibody (700182) in ICC/IF

Product Details

700182

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!