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Invitrogen
Caspase 4 Polyclonal Antibody
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Caspase 4 Antibody (PA5-18300) in WB
Western blot was performed using Anti-Caspase 4 Polyclonal Antibody (Product # PA5-18300) and a ~45 kDa band corresponding to Caspase 4 was observed in cell lines tested along with few uncharacterized bands at ~80, 160 kDa. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), Raw 264.7 (Lane 2), U-937 (Lane 3), U-2 OS (Lane 4), HeLa (Lane 5) and HEK-293 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB230... View More
Product Details
PA5-18300
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (CKERAEEIYPIKERN) corresponding to the internal amino acids of CASP4 (aa 118-133).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10987547
Product Specific Information
This antibody is predicted to react with bovine, canine, feline and mouse based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptotic cysteine protease Mih1/TX; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-4; ICE and Ced-3 homolog 2; ICE(rel)-II; ICH-2; Mih1; protease ICH-2; Protease TX
Gene Aliases: CASP4; ICE(rel)II; ICEREL-II; ICH-2; ICH2; Mih1; Mih1/TX; TX
UniProt ID: (Human) P49662
Entrez Gene ID: (Human) 837
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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