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Invitrogen
Caspase 4 Polyclonal Antibody
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FIGURE: 1 / 9
Caspase 4 Antibody (PA5-95498) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Caspase 4 in A431 cell using Caspase 4 Polyclonal Antibody (Product # PA5-95498). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dil... View More
Product Details
PA5-95498
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Caspase 4 recombinant protein (Position: H111-R194).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807300
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human COLO-320 whole cell, human SW620 whole cell, human HepG2 whole cell. IHC: human colon cancer tissue, human mammary cancer tissue. ICC/IF: A431 cell. Flow: SiHa cell, PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptotic cysteine protease Mih1/TX; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-4; ICE and Ced-3 homolog 2; ICE(rel)-II; ICH-2; Mih1; protease ICH-2; Protease TX
Gene Aliases: CASP4; ICE(rel)II; ICEREL-II; ICH-2; ICH2; Mih1; Mih1/TX; TX
UniProt ID: (Human) P49662
Entrez Gene ID: (Human) 837
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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