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Catalase Antibody (PA5-95349) in ICC/IF
Immunocytochemistry analysis of Catalase using anti-Catalase antibody (Product # PA5-95349) . Catalase was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Catalase antibody (Product # PA5-95349) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More
Product Details
PA5-95349
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Catalase recombinant protein (Position: E344-L527).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807152
Product Specific Information
Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human HL-60 whole cell, human THP-1 whole cell, human U937 whole cell, human HepG2 whole cell, human K562 whole cell, human HCCT tissue, human HCCP tissue, rat liver tissue, rat kidney tissue, rat lung tissue, rat RH35 whole cell, mouse liver tissue, mouse kidney tissue, mouse lung tissue. IHC: mouse liver tissue, rat liver tissue, human liver cancer tissue. ICC/IF: A431 cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Catalase; catalase 1; CTA1; HGNC:1516; MGC138422; MGC138424
Gene Aliases: 2210418N07; Cas-1; Cas1; CAT; Cat01; Catl; Cs-1; CS1
UniProt ID: (Human) P04040, (Rat) P04762, (Mouse) P24270
Entrez Gene ID: (Human) 847, (Rat) 24248, (Mouse) 12359
response to reactive oxygen species
osteoblast differentiation
ureteric bud development
response to hypoxia
purine nucleotide catabolic process
triglyceride metabolic process
aging
cholesterol metabolic process
aerobic respiration
response to light intensity
UV protection
response to ozone
response to lead ion
positive regulation of phosphatidylinositol 3-kinase signaling
response to activity
response to inactivity
hemoglobin metabolic process
negative regulation of NF-kappaB transcription factor activity
response to estradiol
response to insulin
response to vitamin A
response to vitamin E
response to L-ascorbic acid
response to drug
response to hydrogen peroxide
hydrogen peroxide catabolic process
negative regulation of apoptotic process
response to ethanol
response to cadmium ion
positive regulation of NF-kappaB transcription factor activity
protein tetramerization
protein homotetramerization
positive regulation of cell division
response to hyperoxia
response to fatty acid
cellular response to growth factor stimulus
response to phenylpropanoid
cellular oxidant detoxification
kidney development
response to oxidative stress
response to radiation
response to UV
response to toxic substance
oxidation-reduction process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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