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Invitrogen
Catenin alpha-1 Monoclonal Antibody (10I2)
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Catenin alpha-1 Antibody (MA5-49204) in IHC (P)
Immunohistochemistry analysis of Catenin alpha-1 in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with Catenin alpha-1 Monoclonal antibody (Product # MA5-49204) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat... View More
Product Details
MA5-49204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10I2
Immunogen
E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074862
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human U20S whole cell, human U-87MG whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell. IHC: human mammary cancer tissue. Flow: Jurkat cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; Catenin alpha-1; catenin, alpha 1; Renal carcinoma antigen NY-REN-13
Gene Aliases: 2010010M04Rik; AA517462; AI988031; CAP102; Catna1; CTNNA1; MDPT2
UniProt ID: (Human) P35221, (Mouse) P26231
Entrez Gene ID: (Human) 1495, (Rat) 307505, (Mouse) 12385
ovarian follicle development
actin filament organization
cell adhesion
establishment or maintenance of cell polarity
negative regulation of neuroblast proliferation
aging
male gonad development
gap junction assembly
axon regeneration
adherens junction organization
cellular protein localization
odontogenesis of dentin-containing tooth
apical junction assembly
response to estrogen
positive regulation of smoothened signaling pathway
positive regulation of muscle cell differentiation
protein heterooligomerization
cellular response to indole-3-methanol
epithelial cell-cell adhesion
negative regulation of cell motility
negative regulation of integrin-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
response to organic cyclic compound
regulation of cell proliferation
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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