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Invitrogen
Cathepsin L Recombinant Rabbit Monoclonal Antibody (221)
FIGURE: 1 / 4
Cathepsin L Antibody (MA5-29206) in WB
Western Blot using Cathepsin L Recombinant Rabbit Monoclonal Antibody (221) (Product # MA5-29206) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: A549 Whole Cell Lysate, Lane C: HCT116 Whole Cell Lysate, Lane D: U87MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 25 kDa.
Product Details
MA5-29206
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
221
Immunogen
Recombinant Human Cathepsin L/CTSL/CTSL1 protein (Met1-Val333)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785117
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Cathepsin L/CTSL/CTSL1.
Target Information
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cathepsin L1; Major excreted protein; MEP; Procathepsin L
Gene Aliases: CATL; CTSL; CTSL1; MEP
UniProt ID: (Human) P07711
Entrez Gene ID: (Human) 1514
toll-like receptor signaling pathway
adaptive immune response
proteolysis
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
extracellular matrix disassembly
collagen catabolic process
proteolysis involved in cellular protein catabolic process
macrophage apoptotic process
cellular response to thyroid hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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