Invitrogen

Cdc25A Monoclonal Antibody (DCS121)

3 Published Figures

9 References

View all (34) Cdc25A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

Cdc25A Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 3

Cdc25A Antibody (MA1-12293) in IHC

BMC cancer 2010 - Figure 3 Expression of CDC25 protein in vulvar cancer cell line . Immunohistochemical staining of CDC25A (a), CDC25B (b), CDC25C (c) and phospho-CDC25C (Ser216) (d) in CAL-39 cell line. Western blot analysis of CDC25s cellular localization in CAL-39 cells (e). Cell fractions of cytoplasm (lane 1) and nucleus (lane 2) were immunoblotted with Tubulin and Lamin B antibodies to verify the purity of subcellular ... View More

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Product Details

MA1-12293

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunoprecipitation (IP)

1-10 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

DCS121

Immunogen

Purified recombinant CDC25A protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1073533

Target Information

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

β-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage.

Pathogens (Basel, Switzerland)

Snow JA,Murthy V,Dacus D,Hu C,Wallace NA

Published figure using Cdc25A monoclonal antibody (Product # MA1-12293) in Western Blot

Tue Nov 26 00:00:00 UTC 2019

Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest.

International journal of cancer

Neumann J,Yang Y,Köhler R,Giaisi M,Witzens-Harig M,Liu D,Krammer PH,Lin W,Li-Weber M

MA1-12293 was used in western blot to characterize suppression of tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest via mangrove dolabrane-type of diterpenes tagalsins

Tue Dec 01 00:00:00 UTC 2015

Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes.

BMC cancer

Wang Z,Trope CG,Flørenes VA,Suo Z,Nesland JM,Holm R

Published figure using Cdc25A monoclonal antibody (Product # MA1-12293) in Immunohistochemistry

Tue May 25 00:00:00 UTC 2010

The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress.

Molecular cell

Zhang YW,Brognard J,Coughlin C,You Z,Dolled-Filhart M,Aslanian A,Manning G,Abraham RT,Hunter T

MA1-12293 was used in western blot to investigate the regulatory effect of Fbx6 on Chk1 turnover and on the cellular sensitivity to replication stress

Fri Aug 28 00:00:00 UTC 2009

Dual regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control.

The Journal of biological chemistry

Demidova AR,Aau MY,Zhuang L,Yu Q

MA1-12293 was used in western blot to study the regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control

Fri Feb 13 00:00:00 UTC 2009

The let-7 microRNA represses cell proliferation pathways in human cells.

Cancer research

Johnson CD,Esquela-Kerscher A,Stefani G,Byrom M,Kelnar K,Ovcharenko D,Wilson M,Wang X,Shelton J,Shingara J,Chin L,Brown D,Slack FJ

MA1-12293 was used in western blot to study the role of let-7 microRNA in repressing human cell proliferation pathways

Wed Aug 15 00:00:00 UTC 2007

How adeno-associated virus Rep78 protein arrests cells completely in S phase.

Proceedings of the National Academy of Sciences of the United States of America

Berthet C,Raj K,Saudan P,Beard P

MA1-12293 was used in western blot to determine how adeno-associated virus Rep78 protein arrests cells completely in S phase

Tue Sep 20 00:00:00 UTC 2005

Estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of the breast cancer cell line T47D.

Proceedings of the National Academy of Sciences of the United States of America

Ström A,Hartman J,Foster JS,Kietz S,Wimalasena J,Gustafsson JA

MA1-12293 was used in western blot to study the mechanism by which estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of breast cancer cells

Tue Feb 10 00:00:00 UTC 2004

Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents.

The Journal of biological chemistry

Xiao Z,Chen Z,Gunasekera AH,Sowin TJ,Rosenberg SH,Fesik S,Zhang H

MA1-12293 was used in western blot to investigate the role of Chk1 in the S and G2 arrests in response to DNA-damaging agents

Fri Jun 13 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: CDC25 isoform A1-CAG; CDC25A2-CAG isoform; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1

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Gene Aliases: CDC25A; CDC25A2

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UniProt ID: (Human) P30304

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Entrez Gene ID: (Human) 993

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding protein kinase binding

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle DNA replication mitotic nuclear division cell proliferation response to radiation cellular response to UV peptidyl-tyrosine dephosphorylation cell division regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc25A Monoclonal Antibody (DCS121)

Cdc25A Antibody (MA1-12293) in IHC

Product Details

MA1-12293

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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