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Invitrogen
Cdc25B Polyclonal Antibody
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FIGURE: 1 / 5
Cdc25B Antibody (PA5-17759) in ICC/IF
Immunofluorescence analysis of CDC25B was performed using 70% confluent log phase HeLa and serum starved HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CDC25B Polyclonal Antibody (Product # PA5-17759) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
Product Details
PA5-17759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues at the carboxy terminus of human cdc25B
Conjugate
Form
Liquid
Concentration
278 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11003214
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2
Gene Aliases: AI604853; CDC25B; CDC25HU2; Cdc25m2
UniProt ID: (Human) P30305, (Mouse) P30306, (Rat) P48966
Entrez Gene ID: (Human) 994, (Mouse) 12531, (Rat) 171103
G2/M transition of mitotic cell cycle
mitotic cell cycle
oocyte maturation
protein phosphorylation
mitotic nuclear division
female meiosis I
positive regulation of cell proliferation
positive regulation of cytokinesis
peptidyl-tyrosine dephosphorylation
positive regulation of protein kinase activity
positive regulation of mitotic cell cycle
cell division
mitotic M phase
protein dephosphorylation
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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