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Invitrogen
Cdc42EP3 Polyclonal Antibody
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FIGURE: 1 / 4
Cdc42EP3 Antibody (PA5-101503) in ICC/IF
Immunofluorescent analysis of Cdc42EP3 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Cdc42EP3 polyclonal antibody (Product # PA5-101503) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101503
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CDC42EP3(Accession Q9UKI2), corresponding to amino acid residues I31-H81.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850938
Product Specific Information
Antibody detects endogenous levels of total BORG2.
Target Information
CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Binder of Rho GTPases 2; CDC42 effector protein (Rho GTPase binding) 3; Cdc42 effector protein 3; CRIB-containing BORG2 protein; MSE55-related Cdc42-binding protein; MSE55-related protein
Gene Aliases: 3200001F04Rik; AA986861; BORG2; CDC42EP3; CEP3; UB1
UniProt ID: (Human) Q9UKI2, (Mouse) Q9CQC5
Entrez Gene ID: (Human) 10602, (Mouse) 260409, (Rat) 313838
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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