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Invitrogen
Cdc5L Recombinant Rabbit Monoclonal Antibody (22H12L1)
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FIGURE: 1 / 6
Cdc5L Antibody (702831) in ICC/IF
For immunofluorescence analysis,HeLa cells were fixed and permeabilized for detection of endogenous CDC5L using Anti-CDC5L Recombinant Rabbit Monoclonal Antibody (Product # 702831, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000).Panel a) shows representative cells that were stained for detection and localization of CDC5L protein (green), panel b is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). P... View More
Product Details
702831
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
22H12L1
Immunogen
Protein corresponding to human CDC5L [aa1-aa200]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734799
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit, Cat, Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CDC5 cell division cycle 5-like; Cdc5-like protein; Cdc5-related protein; Cell division cycle 5-like protein; cell division cycle 5-related protein; dJ319D22.1 (CDC5-like protein); hCDC5; Pombe cdc5-related protein
Gene Aliases: 1200002I02Rik; AA408004; CDC5; CDC5-LIKE; CDC5L; CEF1; dJ319D22.1; KIAA0432; PCDC5RP
UniProt ID: (Human) Q99459, (Mouse) Q6A068
Entrez Gene ID: (Human) 988, (Mouse) 71702
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor recruiting
transcription corepressor binding
DNA binding
protein binding
protein phosphatase 1 binding
protein kinase binding
leucine zipper domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
WD40-repeat domain binding
chromatin binding
RNA binding
protein domain specific binding
mitotic cell cycle
mRNA splicing, via spliceosome
DNA repair
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell differentiation
cellular response to fibroblast growth factor stimulus
cellular response to interleukin-2
signal transduction involved in DNA damage checkpoint
cellular response to wortmannin
cellular response to nerve growth factor stimulus
cellular response to prolactin
regulation of transcription, DNA-templated
mRNA processing
cellular response to DNA damage stimulus
cell cycle
RNA splicing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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