Invitrogen

Cdc73 Polyclonal Antibody

Cdc73 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Cdc73 Antibody (PA5-26189) in ICC/IF

Immunocytochemistry analysis of Cdc73 in HeLa cells. Samples were incubated in Cdc73 polyclonal antibody (Product # PA5-26189) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Cdc73 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

PA5-26189

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:25

Immunocytochemistry (ICC/IF)

1:10-1:50

View 3 publications 3 publications

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 132-161 amino acids from the central region of human CDC73

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2543689

Product Specific Information

This antibody is predicted to react with chicken, mouse and rat based on sequence homology.

Target Information

Cdc73 is a tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. Cdc73 is part of the PAF1 complex, a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9. PAF1 was first purified from yeast in association with RNA polymerase II and is believed to function in transcription elongation. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. In addition to coordinating events during transcription (initiation, promoter clearance, and elongation), hPAF also coordinates events in RNA quality control. Cdc73 may also be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stress-mediated aggregation of disease-associated proteins in amyloid bodies.

Scientific reports

Chandhok S,Pereira L,Momchilova EA,Marijan D,Zapf R,Lacroix E,Kaur A,Keymanesh S,Krieger C,Audas TE

PA5-26189 was used in Immunocytochemistry-immunofluorescence to demonstrate that pathogenic proteins associated with Alzheimer's disease, diabetes, AL/AA amyloidosis, and amyotrophic lateral sclerosis can aggregate within stress-inducible physiological amyloid-based structures, termed amyloid bodies (A-bodies).

Sat Sep 02 00:00:00 UTC 2023

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

PA5-26189 was used in Immunocytochemistry, Western Blot to show that solid-like amyloid bodies coordinate local nuclear protein synthesis during stress.

Tue Feb 16 00:00:00 UTC 2021

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

PA5-26189 was used in Immunocytochemistry, Western Blot to show that solid-like amyloid bodies coordinate local nuclear protein synthesis during stress.

Tue Feb 16 00:00:00 UTC 2021

Stress-specific aggregation of proteins in the amyloid bodies.

FEBS letters

Marijan D,Tse R,Elliott K,Chandhok S,Luo M,Lacroix E,Audas TE

PA5-26189 was used in Immunocytochemistry-immunoflourescence to propose that different environmental conditions induce the formation of A-body subtypes containing distinct protein residents.

Fri Nov 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: cell division cycle 73 Paf1/RNA polymerase II complex component-like protein; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog; Cell division cycle protein 73 homolog; Hyperparathyroidism 2 protein; Paf1/RNA polymerase II complex component; Parafibromin

View more View less

Gene Aliases: C1orf28; CDC73; FIHP; HPTJT; HRPT1; HRPT2; HYX

View more View less

UniProt ID: (Human) Q6P1J9

View more View less

Entrez Gene ID: (Human) 79577

View more View less

Function(s)

RNA polymerase II core binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter endodermal cell fate commitment transcription elongation from RNA polymerase II promoter mRNA polyadenylation cell cycle negative regulation of cell proliferation histone monoubiquitination Wnt signaling pathway stem cell population maintenance positive regulation of Wnt signaling pathway positive regulation of mRNA 3'-end processing protein destabilization positive regulation of transcription elongation from RNA polymerase II promoter histone H2B ubiquitination negative regulation of myeloid cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of fibroblast proliferation negative regulation of epithelial cell proliferation cellular response to lipopolysaccharide beta-catenin-TCF complex assembly negative regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Cdc73 Polyclonal Antibody

Cdc73 Antibody (PA5-26189) in ICC/IF

Product Details

PA5-26189

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!