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Invitrogen
Centrin 3 Polyclonal Antibody
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FIGURE: 1 / 4
Centrin 3 Antibody (PA5-35865) in ICC/IF
Centrin3 antibody detects Centrin3 protein at centrosome by immunofluorescent analysis. Sample: A549 cells were fixed in 100% MeOH for 5 min. Green: Centrin3 protein stained by Centrin3 antibody (Product # PA5-35865) diluted at 1:1,000. Red: alpha Tubulin Polyclonal Antibody [B-5-1-2] (Product # MA1-25054) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-35865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Centrin 3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2553175
Product Specific Information
Recommended positive controls: A549, H1299, HCT-116, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (89%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC31 yeast homolog; centrin 3; centrin, EF-hand protein, 3 (CDC31 homolog, yeast); Centrin-3; EF-hand superfamily member
Gene Aliases: CDC31; CEN3; CETN3; MmCEN3
UniProt ID: (Human) O15182, (Mouse) O35648
Entrez Gene ID: (Human) 1070, (Mouse) 12626, (Rat) 170895
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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