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Invitrogen
Chicken anti-Human IgM Secondary Antibody, HRP
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FIGURE: 1 / 3
Human IgM Secondary Antibody (SA1-72053) in WB
Western blot was performed using Chicken anti-Human IgM Secondary Antibody, HRP (Product # SA1-72053) and an ~80 kDa band corresponding to Human IgM Heavy Chain was observed in Raji, Ramos and Daudi but not in IM-9, ARH-77, MOLT-4 and Jurkat. Whole cell extracts (30 µg) of IM-9 (Lane 1), ARH-77 (Lane 2), Raji (Lane 3), Ramos (Lane 4), Daudi (Lane 5), MOLT-4 (Lane 6) and Jurkat (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane ... View More
Product Details
SA1-72053
Applications
Tested Dilution
Western Blot (WB)
1:1000-1:2000
ELISA (ELISA)
1:10,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified pooled normal human IgM.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.1% chloroacetamide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_923383
Target
IgM
Antibody Form
Whole Antibody
Product Specific Information
SA1-72053 detects Human IgM in human samples.
SA1-72053 has been successfully used in ELISA and Western blot procedures.
The SA1-72053 immunogen is from purified pooled normal human IgM.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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