Proteintech

Cingulin Polyclonal Antibody

Cingulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Cingulin Antibody (21369-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 21369-1-AP (Cingulin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cingulin Antibody in Immunoprecipitation (IP)

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Product Details

21369-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cingulin Fusion Protein Ag15843 (1-350 aa encoded by BC146657)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAEPRGPVD HGVQIRFITE PVSGAEMGTL RRGGRRPAKD ARASTYGVAV RVQGIAGQPF VVLNSGEKGG DSFGVQIKGA NDQGASGALS SDLELPENPY SQVKGFPAPS QSSTSDEEPG AYWNGKLLRS HSQASLAGPG PVDPSNRSNS MLELAPKVAS PGSTIDTAPL SSVDSLINKF DSQLGGQARG RTGRRTRMLP PEQRKRSKSL DSRLPRDTFE ERERQSTNHW TSSTKYDNHV GTSKQPAQSQ NLSPLSGFSR SRQTQDWVLQ SFEEPRRSAQ DPTMLQFKST PDLLRDQQEA APPGSVDHMK ATIYGILREG SSESETSVRR KVSLVLEKMQ PLVMVSSGST K (1-350 aa encoded by BC146657)

Target Information

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.

Mucosal immunology

Ou J,Guan X,Wang J,Wang T,Zhang B,Li R,Xu H,Hu X,Guo XK

Tue Feb 01 00:00:00 UTC 2022

Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.

Mucosal immunology

Ou J,Guan X,Wang J,Wang T,Zhang B,Li R,Xu H,Hu X,Guo XK

Tue Feb 01 00:00:00 UTC 2022

miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.

Cancers

Zhang X,Li T,Han YN,Ge M,Wang P,Sun L,Liu H,Cao T,Nie Y,Fan D,Guo H,Wu K,Zhao X,Lu Y

Mon Nov 15 00:00:00 UTC 2021

miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.

Cancers

Zhang X,Li T,Han YN,Ge M,Wang P,Sun L,Liu H,Cao T,Nie Y,Fan D,Guo H,Wu K,Zhao X,Lu Y

Mon Nov 15 00:00:00 UTC 2021

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Nature communications

Liu Y,Zhang Y,Li T,Han J,Wang Y

Thu Jan 30 00:00:00 UTC 2020

Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.

Biology of reproduction

Xiong Z,Wang C,Wang Z,Dai H,Song Q,Zou Z,Xiao B,Zhao AZ,Bai X,Chen Z

Sat Dec 01 00:00:00 UTC 2018

Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.

Nature communications

Mangan AJ,Sietsema DV,Li D,Moore JK,Citi S,Prekeris R

Wed Aug 03 00:00:00 UTC 2016

Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.

Nature communications

Mangan AJ,Sietsema DV,Li D,Moore JK,Citi S,Prekeris R

Wed Aug 03 00:00:00 UTC 2016

Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate.

Cellular signalling

Ducommun S,Deak M,Sumpton D,Ford RJ,Núñez Galindo A,Kussmann M,Viollet B,Steinberg GR,Foretz M,Dayon L,Morrice NA,Sakamoto K

Fri May 01 00:00:00 UTC 2015

Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate.

Cellular signalling

Ducommun S,Deak M,Sumpton D,Ford RJ,Núñez Galindo A,Kussmann M,Viollet B,Steinberg GR,Foretz M,Dayon L,Morrice NA,Sakamoto K

Fri May 01 00:00:00 UTC 2015

Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate.

Cellular signalling

Ducommun S,Deak M,Sumpton D,Ford RJ,Núñez Galindo A,Kussmann M,Viollet B,Steinberg GR,Foretz M,Dayon L,Morrice NA,Sakamoto K

Fri May 01 00:00:00 UTC 2015

Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate.

Cellular signalling

Ducommun S,Deak M,Sumpton D,Ford RJ,Núñez Galindo A,Kussmann M,Viollet B,Steinberg GR,Foretz M,Dayon L,Morrice NA,Sakamoto K

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Cingulin

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Gene Aliases: 6330408J11Rik; CGN; KIAA1319

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UniProt ID: (Human) Q9P2M7

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Entrez Gene ID: (Human) 57530, (Mouse) 70737, (Rat) 310655

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Function(s)

motor activity actin binding protein binding cadherin binding involved in cell-cell adhesion microtubule binding

Process(es)

transforming growth factor beta receptor signaling pathway biological_process cell-cell adhesion microtubule cytoskeleton organization epithelial cell morphogenesis bicellular tight junction assembly metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cingulin Polyclonal Antibody

Cingulin Antibody (21369-1-AP) in ICC/IF

Product Details

21369-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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