Invitrogen

Claudin 15 Polyclonal Antibody

10 Published Figures

View all (5) Claudin 15 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (2)
Published Figures (10)

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 12

Claudin 15 Antibody (38-9200) in IHC

Immunofluorescent co-labeling (yellow) of claudin-15 (red) and ZO-1 (green) in mouse ileum tissue using Rb anti-Claudin-15 (Product # 38-9200). Image courtesy of Jennifer Holmes and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Immunohistochemistry (IHC)

Claudin 15 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Claudin 15 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Claudin 15 Antibody in Western Blot (WB)

Product Image Gallery

Claudin 15 Antibody in Immunohistochemistry (IHC) — Immunofluorescent co-labeling (yellow) of claudin-15 (red) and ZO-1 (green) in mouse ileum tissue using Rb anti-Claudin-15 (Product # 38-9200). Image courtesy of Jennifer Holmes and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

Claudin 15 Antibody in Immunohistochemistry (IHC) — Immunofluorescent staining of (A) Anti-Claudin 15; (B) Claudin 15 (red), ZO-1 (Green) co localization (yellow); (C) Anti-Claudin 15; (D) Claudin 15 (red), ZO-1 (green) -- C and D (blocked by fusion protein) using Rb anti-Claudin-15 (Product # 38-9200).

Claudin 15 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Segregation enables paracellular ion flux over the TJ meshwork. a Representative confocal images of MDCKII QKO cells stably expressing (sT) FLAG-Cldn2, FLAG-Cldn10a, and FLAG-Cldn2+FLAG-Cldn10a. Single claudin expressing cells were immunostained with anti-Cldn2 (magenta; 2 nd -Atto647N) or anti-Cldn10 (magenta; 2 nd -Atto647N) and anti-ZO-1 (yellow; 2 nd -AF594) antibody. Double claudin expressing cells were immunostained with anti-Cldn2 (magenta; 2 nd -Atto647N) and anti-Cldn10 (yellow; 2 nd -AF594). The white rectangle indicates the region of interest for the magnification in ( b ). b Magnification of FLAG-Cldn2+FLAG-Cldn10a expressing cells from ( a ). White arrows point out differently sized TJ parts that contain only Cldn10a. c Representative confocal images of MDCKII QKO sT FLAG-Cldn3, FLAG-Cldn15 and FLAG-Cldn3+FLAG-Cldn15. Single and double claudin expressing cells were stained with anti-Cldn3 (magenta; 2 nd -Atto647N) or anti-Cldn15 (magenta; 2 nd -Atto647N) and anti-ZO-1 (yellow; 2 nd -AF594) antibody. d Summary of the TER (ohm*cm 2 ), fluorescein flux (10 -6 cm/s) and PNa/PCl ratio from the measured cell lines: MDCKII, MDCKII QKO and MDCKII QKO sT FLAG-tagged Cldn2, Cldn3, Cldn10a, Cldn15 and MDCKII QKO sT FLAG-tagged Cldn2 + Cldn10a and FLAG-tagged Cldn3 + Cldn15. Data represent the mean +- SD from 3 to 5 independent experiments. Single data points are shown in Supplementary Fig. 12 . e Absolute permeability for sodium (PNa; magenta bars) and chloride (PCl; cyan b

Claudin 15 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Claudins form characteristic copolymers including segregation of channel-forming claudins from Cldn3. a Scheme is illustrating predicted claudin-claudin organization patterns. Two claudins (yellow and magenta) that are compatible with each other tightly colocalize in the TJ (pink). Incompatible claudins separate into different strands or even larger claudin-specific parts. This separation might facilitate permeability of specific ions (cyan) over the TJ meshwork. b Representative STED images of TJ-like meshwork of the observed five organization patterns formed by the indicated co-expressed claudins (SNAP-tagged (yellow; BG-Atto590) and YFP-tagged (magenta; alpha-GFP-NB-Atto647N)) in fixed COS-7 cells are shown. Tight colocalization: intermixing of mesh-forming claudins, integration of non-mesh-forming with a mesh-forming claudin or induction (de novo mesh-forming of co-expressed non-mesh-forming claudins). Separated claudins: segregation and exclusion. c Pearson correlation analysis of Cldn3 co-expressed with barrier-forming (gray) or channel-forming (magenta) claudins. Co-expression of Cldn3 with Cldn3 (yellow) served as positive control. Data represent the mean +- SD. Every n represents the Pearson of one TJ-like meshwork. n (Cldn3 + Cldn3) = 55; n (Cldn3 + Cldn1) = 54; n (Cldn3 + Cldn5) = 20; n (Cldn3 + Cldn6) = 20; n (Cldn3 + Cldn7) = 17; n (Cldn3 + Cldn9) = 15; n (Cldn3 + Cldn14) = 19; n (Cldn3 + Cldn19b) = 25; n (Cldn3 + Cldn2) = 36; n (Cldn3 + Cldn10a) = 30; n (Cldn3 +

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — 3 FIGURE Dapiglutide promotes cation selectivity via expansion of cation-selective claudins in the epithelium. (A) Biionic dilution potentials were measured in the Ussing chamber by replacing 50% NaCl in the basal solution with counter-cation chloride in increasing size, n = 5-6 per group. Mean +- SEM, * p < 0.05 versus ICR + vehicle, SS p < 0.05 for ICR + vehicle versus ICR + dapiglutide. (B) Upper panel: representative western blot of claudin-2 protein of jejunum tissue. Lower panel: corresponding densitometric quantification, n = 4-5 per group, mean +- SEM, * p < 0.05, ** p < 0.01. (C) Upper panel: representative western blot of claudin-15 protein of jejunum tissue. Lower panel: corresponding densitometric quantification, n = 4-5 per group, mean +- SEM. (D) Immunofluorescence images of jejunum sections: claudin staining in green, F-actin in red, and nuclei in blue. Scale bar: 50 mum. White arrows indicate the extension of the indicated claudin along the crypt-villus axis

Claudin 15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5 Claudin-15 and ion transport are modulated in Hnf4alpha colon null mutants. (A) qRT-PCR of claudin-4, -8 and -15 at various time points in mutants (Open bars) relative to controls (Black bars). Expression levels are shown as mean values (+- SEM) relative to controls at each time point (3-14 mice per group) and were normalized with the Tbp housekeeping gene. (B) Immunoblotting confirmed modulated protein levels of claudin-4, -8 and -15 at 12 months in mutants (n = 3, open bars) and relative controls (n = 3, black bars) normalized to stable expression of E-Cadherin (Cdh1). (C) Quantification of band intensities of each claudin (means +- SEM; n = 3 or 4 for each group) relative to Cdh1. (D) Indirect immunofluorescence (FITC) of claudin-4, -8 and -15 in 3 month-old animals in proximal colon (representative of 4 pairs). Nuclei were counterstained with DAPI (blue) and merged with FITC (green). Scale bars, 50 um. (E) Claudin-15 expression is lost in the upper half of the colon epithelium in mutants (Open bar) relative to controls (Black bar). (F) Colonic permeability to 51 Cr-EDTA measured in colon tissues from 2 month-old mice (5 mice per group). (G) Baseline short circuit current (Isc) measured in colon tissues from 2 month-old mice (5 mice per group). Represented as mean values +- SEM, * P <=.05, ** P <=.01, *** P <=.001.

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Claudin 15 Antibody in Western Blot (WB) — Figure 6 The claudin-15 gene is a direct target of Hnf4alpha. (A) EMSA of 4 Hnf4alpha binding sites found in the 1 kb mouse claudin-15 promoter performed with 293T cells transfected with pBabe Hnf4alpha1 vector and 293T nuclear extracts as negative controls. Shifts (white arrow head) are compared to previously published APOC3 and mutated APOC3 (APOC3m) for control binding site. NS is non-specific binding (grey arrow head). Supershifts (black arrow head) were done with Hnf4alpha antibody (sc-6556). (B) ChIP assays of the 4 putative sites performed on epithelial cells from adult mouse colon. (C) qRT-PCR of claudin-15 on IEC6 cells infected with pBabe Hnf4alpha as compared to control vector pBabe (n = 4). (D) Western blot of 3 stable T84 cell populations expressing mouse claudin-15. (E) Conductance measured for three consecutive days after the reach of confluence on three populations of T84 cells expressing mouse claudin-15 or control plenti eGFP vector. Represented as mean values+-SEM, *** P <=.001.

Product Details

38-9200

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse Claudin-15 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533391

Target Information

Plays a major role in tight junction specific obliteration of the intercellular space, through calcium independent cell adhesion activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The dual GLP-1 and GLP-2 receptor agonist dapiglutide promotes barrier function in murine short bowel.

Annals of the New York Academy of Sciences

Reiner J,Thiery J,Held J,Berlin P,Skarbaliene J,Vollmar B,Jaster R,Eriksson PO,Lamprecht G,Witte M

Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Mon Aug 01 00:00:00 UTC 2022

The dual GLP-1 and GLP-2 receptor agonist dapiglutide promotes barrier function in murine short bowel.

Annals of the New York Academy of Sciences

Reiner J,Thiery J,Held J,Berlin P,Skarbaliene J,Vollmar B,Jaster R,Eriksson PO,Lamprecht G,Witte M

Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Mon Aug 01 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

38-9200 was used in Immunohistochemistry (Frozen), Immunocytochemistry to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

38-9200 was used in Immunohistochemistry (Frozen), Immunocytochemistry to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

38-9200 was used in Immunohistochemistry (Frozen), Immunocytochemistry to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

38-9200 was used in Immunohistochemistry (Frozen), Immunocytochemistry to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

38-9200 was used in Immunohistochemistry (Frozen), Immunocytochemistry to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Loss of NKCC1 function increases epithelial tight junction permeability by upregulating claudin-2 expression.

American journal of physiology. Cell physiology

Koumangoye R,Penny P,Delpire E

38-9200 was used in Western Blot to investigate if Na+-K+-2Cl- cotransporter NKCC1 regulates tight junction protein expression and loss of NKCC1 function affects epithelial barrier integrity.

Sat Oct 01 00:00:00 UTC 2022

Investigation of murine host sex as a biological variable in epithelial barrier function and muscle contractility in human intestinal organoids.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Beanland BT,McNeill EP,Sequeira DJ,Xue H,Shroyer NF,Speer AL

38-9200 was used in Immunohistochemistry (Frozen), Western Blot to investigate intestinal organdie use for epithelial barrier function and muscle contractility modelling.

Tue Nov 01 00:00:00 UTC 2022

Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice.

The Journal of clinical investigation

Raju P,Shashikanth N,Tsai PY,Pongkorpsakol P,Chanez-Paredes S,Steinhagen PR,Kuo WT,Singh G,Tsukita S,Turner JR

38-9200 was used in Western Blot, Immunohistochemistry (Paraffin) to define the impact of claudin-2 channels on disease using mice models.

Thu Oct 01 00:00:00 UTC 2020

Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice.

The Journal of clinical investigation

Raju P,Shashikanth N,Tsai PY,Pongkorpsakol P,Chanez-Paredes S,Steinhagen PR,Kuo WT,Singh G,Tsukita S,Turner JR

38-9200 was used in Western Blot, Immunohistochemistry (Paraffin) to define the impact of claudin-2 channels on disease using mice models.

Thu Oct 01 00:00:00 UTC 2020

Differential regulation of claudin-2 and claudin-15 expression in children and adults with malabsorptive disease.

Laboratory investigation; a journal of technical methods and pathology

Ong MLDM,Yeruva S,Sailer A,Nilsen SP,Turner JR

38-9200 was used in Immunohistochemistry to conclude that contributions of claudin-2 and claudin-15 to pathophysiology of and responses to diarrhea in children and adults with GVHD and CVID differ from those in CD and IBD.

Sun Mar 01 00:00:00 UTC 2020

EspF is crucial for Citrobacter rodentium-induced tight junction disruption and lethality in immunocompromised animals.

PLoS pathogens

Xia X,Liu Y,Hodgson A,Xu D,Guo W,Yu H,She W,Zhou C,Lan L,Fu K,Vallance BA,Wan F

38-9200 was used in Western Blotting to identify a critical pathophysiological function for EspF during Citrobacter rodentium infection in immunocompromised animals.

Sat Jun 01 00:00:00 UTC 2019

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Tissue barriers

Chen H,Lu R,Zhang YG,Sun J

38-9200 was used in Western Blotting to study the role of the vitamin D receptor in lung barrier integrity.

Mon Apr 01 00:00:00 UTC 2019

IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance.

Cell host & microbe

Tsai PY,Zhang B,He WQ,Zha JM,Odenwald MA,Singh G,Tamura A,Shen L,Sailer A,Yeruva S,Kuo WT,Fu YX,Tsukita S,Turner JR

Published figure using Claudin 15 polyclonal antibody (Product # 38-9200) in Western Blot

Wed Jun 14 00:00:00 UTC 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

38-9200 was used in Immunohistochemistry-immunofluorescence to advance the understanding of the molecular undertakings of obesity associated changes that predispose specific diseases and identify novel preventive and/or therapeutic interventions.

Tue Jul 11 00:00:00 UTC 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

38-9200 was used in Immunohistochemistry-immunofluorescence to advance the understanding of the molecular undertakings of obesity associated changes that predispose specific diseases and identify novel preventive and/or therapeutic interventions.

Tue Jul 11 00:00:00 UTC 2017

Disrupted tight junctions in the small intestine of cystic fibrosis mice.

Cell and tissue research

De Lisle RC

38-9200 was used in immunohistochemistry and western blot to characterize tight junctions in the digestive tract of cystic fibrosis mice

Wed Jan 01 00:00:00 UTC 2014

Disrupted tight junctions in the small intestine of cystic fibrosis mice.

Cell and tissue research

De Lisle RC

38-9200 was used in immunohistochemistry and western blot to characterize tight junctions in the digestive tract of cystic fibrosis mice

Wed Jan 01 00:00:00 UTC 2014

EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.

Developmental biology

Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H

Thu Nov 15 00:00:00 UTC 2012

EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.

Developmental biology

Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H

Thu Nov 15 00:00:00 UTC 2012

Dynamics of claudins expression in colitis and colitis-associated cancer in rat.

Methods in molecular biology (Clifton, N.J.)

Arimura Y,Nagaishi K,Hosokawa M

Fri Nov 04 00:00:00 UTC 2011

Dynamics of claudins expression in colitis and colitis-associated cancer in rat.

Methods in molecular biology (Clifton, N.J.)

Arimura Y,Nagaishi K,Hosokawa M

Fri Nov 04 00:00:00 UTC 2011

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Thu Oct 29 00:00:00 UTC 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Thu Oct 29 00:00:00 UTC 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Thu Oct 29 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Claudin-15

View more View less

Gene Aliases: 2210009B08Rik; BB107105; CLDN15

View more View less

UniProt ID: (Human) P56746, (Mouse) Q9Z0S5, (Rat) D3ZQJ0

View more View less

Entrez Gene ID: (Human) 24146, (Mouse) 60363, (Rat) 304388

View more View less

structural molecule activity identical protein binding

transport ion transport calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support