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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Claudin 18 Antibodies

          Invitrogen

          Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

          Advanced Verification
          21 Published Figures
          27 References
          View all (9) Claudin 18 antibodies

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          Datasheet
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          Datasheet
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          Cite Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

          • Antibody Testing Data (3)
          • Published Figures (21)
          • Advanced Verification (1)
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 25

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          Claudin 18 Antibody (700178) in IHC (P)

          Immunohistochemistry analysis of Claudin-18 was performed on paraffin-embedded human normal stomach and squamous lung carcinoma tissue sections. To expose target proteins, endogenous peroxide blocking followed by heat induced epitope retrieval was performed using Tris-EDTA (pH 9. 0) buffer for 15 minutes. Following antigen retrieval, tissues were blocked in 0. 2% BSA with 0. 1% cold fish skin gelatin in 1X ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Immunohistochemistry (IHC)
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody in Western Blot (WB)
          Claudin 18 Antibody

          Product Details

          700178

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2-3 µg/mL
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 13 publications 13 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          2-4 µg/mL
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Amphibian, Dog, Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          34H14L15

          Immunogen

          A peptide corresponding to amino acids 224-237 of P56856.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2532290

          Product Specific Information

          This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.

          Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: claudin 18-like; Claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J

          View more View less

          Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572

          View more View less

          UniProt ID: (Human) P56856, (Mouse) P56857, (Rat) Q5I0E5

          View more View less

          Entrez Gene ID: (Human) 51208, (Mouse) 56492, (Rat) 315953

          View more View less

          Function(s)
          structural molecule activity identical protein binding
          Process(es)
          calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules negative regulation of bone resorption digestive tract development TNFSF11-mediated signaling pathway negative regulation of protein localization to nucleus negative regulation of osteoclast development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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