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          • Primary Antibodies ›
          • Claudin 3 Antibodies

          Invitrogen

          Claudin 3 Polyclonal Antibody

          Advanced Verification
          81 Published Figures
          273 References
          View all (19) Claudin 3 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite Claudin 3 Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (81)
          • Advanced Verification (1)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 86

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          Claudin 3 Antibody (34-1700) in ICC/IF

          Immunofluorescence analysis of Claudin-3 was performed using 70% confluent log phase NIH:OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Claudin 3 Polyclonal Antibody (Product # 34-1700) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunocytochemistry (ICC/IF)
          Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Claudin 3 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          Claudin 3 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Claudin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Claudin 3 Antibody in Immunohistochemistry (IHC)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody in Western Blot (WB)
          Claudin 3 Antibody

          Product Details

          34-1700

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 104 publications 104 publications

          Immunohistochemistry (IHC)

          -
          View 63 publications 63 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          View 12 publications 12 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 10 publications 10 publications

          Immunocytochemistry (ICC/IF)

          1:100
          View 69 publications 69 publications

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Immunoelectrophoresis (IE)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 11 publications 11 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse

          Published species

          Amphibian, Bovine, Dog, Fish, Goat, Hamster, Human, Mouse, Pig, Plant, Rabbit, Rat, Rhesus monkey, Sheep, Virus, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide derived from the C-terminal region of mouse Claudin-3
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533158

          Product Specific Information

          This antibody is specific for the 22 kDa Claudin-3 protein. Reactivity has been confirmed with mouse liver and lung homogenates, canine MDCK and human MCF-7 cell lysates by western blotting, and with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

          Target Information

          Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; hRVP1; integral membrane protein claudin-3; Rat ventral prostate.1 protein homolog; ventral prostate.1 protein homolog; ventral prostate.1-like protein

          View more View less

          Gene Aliases: AI182374; C7orf1; CLDN3; CPE-R2; CPETR2; HRVP1; mRVP1; RVP1

          View more View less

          UniProt ID: (Human) O15551, (Dog) Q95KM5, (Mouse) Q9Z0G9

          View more View less

          Entrez Gene ID: (Human) 1365, (Dog) 403648, (Mouse) 12739

          View more View less

          Function(s)
          transmembrane signaling receptor activity structural molecule activity identical protein binding protein binding
          Process(es)
          response to hypoxia epithelial cell morphogenesis signal transduction calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules protein homooligomerization protein heterooligomerization bicellular tight junction assembly
          It has to be done as per old AB suggested Products section.
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