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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Claudin 4 Antibodies

          Invitrogen

          Claudin 4 Monoclonal Antibody (3E2C1)

          Advanced Verification
          219 References
          View all (15) Claudin 4 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Claudin 4 Monoclonal Antibody (3E2C1)

          • Antibody Testing Data (3)
          • Advanced Verification (1)
          Claudin 4 Antibody in Western Blot (WB)
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          Claudin 4 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Claudin 4 Antibody (32-9400) in WB

          Western blot was performed using Anti- Claudin 4 Monoclonal Antibody (3E2C1) (Product # 32-9400) and a 18 kDa band corresponding to CLDN4 was observed across the positive cells lines tested (MCF7 and HT-29) and not in the negative models (A549 and HeLa) as reported. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 3), A549 (Lane 2) and HeLa (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB230... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Claudin 4 Antibody in Western Blot (WB)
          Claudin 4 Antibody in Western Blot (WB)
          Claudin 4 Antibody in Western Blot (WB)
          Claudin 4 Antibody

          Product Details

          32-9400

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5 - 3 µg/mL
          View 69 publications 69 publications

          Immunohistochemistry (IHC)

          2-3 µg/mL
          View 35 publications 35 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 31 publications 31 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 10 publications 10 publications

          Immunocytochemistry (ICC/IF)

          -
          View 50 publications 50 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 23 publications 23 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Published species

          Amphibian, Bovine, Dog, Fish, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Virus

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3E2C1

          Immunogen

          Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 594 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533096

          Product Specific Information

          Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.

          For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

          This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.

          Target Information

          Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: claudin 4; Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; h CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein

          View more View less

          Gene Aliases: Cep-r; CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8

          View more View less

          UniProt ID: (Human) O14493, (Dog) A0A8C0N7E5, (Mouse) O35054

          View more View less

          Entrez Gene ID: (Human) 1364, (Dog) 100856416, (Rat) 304407, (Mouse) 12740

          View more View less

          Function(s)
          transmembrane signaling receptor activity structural molecule activity identical protein binding molecular_function protein binding
          Process(es)
          female pregnancy circadian rhythm response to progesterone establishment of skin barrier signal transduction calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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