Invitrogen

Clusterin Alpha Recombinant Rabbit Monoclonal Antibody (5I2Q1)

View all (4) Clusterin Alpha antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-51294

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:10,000-1:80,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5I2Q1

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 314-449 of human Clusterin alpha chain (NP_001822.3).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.98 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093802

Product Specific Information

Immunogen sequence: STNNPSQAKL RRELDESLQV AERLTRKYNE LLKSYQWKML NTSSLLEQLN EQFNWVSRLA NLTQGEDQYY LRVTTVASHT SDSDVPSGVT EVVVKLFDSD PITVTVPVEV SRKNPKFMET VAEKALQEYR KKHREE

Target Information

Isoform 1 of Clusterin prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aging-associated gene 4 protein; aging-associated protein 4; Apo-J; Apolipoprotein J; CLI; Clusterin; Clustrin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; SGP-2; Sulfated glycoprotein 2; testosterone repressed prostate message; testosterone repressed prostate message-2; Testosterone-repressed prostate message 2; TRPM-2

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Gene Aliases: AAG4; AI893575; APO-J; APOJ; CLI; CLU; CLU1; CLU2; D14Ucla3; KUB1; Msgp-2; NA1/NA2; SGP-2; SGP2; SP-40; Sugp-2; TRPM-2; TRPM2

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UniProt ID: (Human) P10909, (Mouse) Q06890

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Entrez Gene ID: (Human) 1191, (Mouse) 12759

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Function(s)
protein binding ATPase activity ubiquitin protein ligase binding protein N-terminus binding chaperone binding misfolded protein binding
Process(es)
cell morphogenesis microglial cell activation cell death positive regulation of cell proliferation protein import endocrine pancreas development central nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of protein homooligomerization positive regulation of tumor necrosis factor production positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation neuron projection morphogenesis protein stabilization positive regulation of NF-kappaB transcription factor activity chaperone-mediated protein complex assembly response to misfolded protein chaperone-mediated protein folding microglial cell proliferation intrinsic apoptotic signaling pathway regulation of beta-amyloid clearance regulation of neuron death positive regulation of neuron death positive regulation of beta-amyloid formation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of beta-amyloid formation regulation of neuronal signal transduction positive regulation of tau-protein kinase activity positive regulation of neurofibrillary tangle assembly positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of intrinsic apoptotic signaling pathway release of cytochrome c from mitochondria platelet degranulation lipid metabolic process complement activation complement activation, classical pathway aging response to light stimulus response to wounding response to virus response to potassium ion reverse cholesterol transport estrous cycle innate immune response cellular response to growth factor stimulus
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