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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Collagen I Antibodies

          Invitrogen

          Collagen I Polyclonal Antibody

          Advanced Verification
          16 Published Figures
          34 References
          View all (26) Collagen I antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Collagen I Polyclonal Antibody

          • Antibody Testing Data (3)
          • Published Figures (16)
          • Advanced Verification (1)
          Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 20

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          Collagen I Antibody (PA1-26204) in IHC (P)

          Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded normal kidney using Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:100 for 4 hours at room temperature. A peroxidase goat anti-rabbit was used as a secondary antibody at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Stain... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Immunocytochemistry (ICC/IF)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Immunocytochemistry (ICC/IF)
          Collagen I Antibody in Immunocytochemistry (ICC/IF)
          Collagen I Antibody in Immunocytochemistry (ICC/IF)
          Collagen I Antibody in Immunohistochemistry (IHC)
          Collagen I Antibody in Immunohistochemistry (IHC)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Western Blot (WB)
          Collagen I Antibody in Flow Cytometry (Flow)
          Collagen I Antibody

          Product Details

          PA1-26204

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          View 13 publications 13 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 1 publication 1 publication

          ELISA (ELISA)

          1:5,000-1:50,000
          -

          Immunoprecipitation (IP)

          1:100
          -

          Dot blot (DB)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Hamster, Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Collagen Type I from human and bovine placenta.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.15 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 8.0, with 150mM NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2260734

          Product Specific Information

          Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.

          Target Information

          Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

          View more View less

          Gene Aliases: AA960264; AI325291; BOS_18945; BOS_4009; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; I79_010913; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

          View more View less

          UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

          View more View less

          Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Rat) 29393, (Chinese hamster) 100759814, (Mouse) 12842, (Chinese hamster) 100766269, (Mouse) 12843, (Rat) 84352

          View more View less

          Function(s)
          extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding molecular_function
          Process(es)
          skeletal system development ossification blood vessel development osteoblast differentiation intramembranous ossification endochondral ossification response to nutrient visual perception sensory perception of sound response to mechanical stimulus positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion protein transport collagen fibril organization positive regulation of cell migration response to nutrient levels response to corticosteroid response to estradiol collagen biosynthetic process protein localization to nucleus tooth mineralization wound healing response to drug response to hydrogen peroxide response to peptide hormone skin development skin morphogenesis cellular response to fibroblast growth factor stimulus tooth eruption positive regulation of transcription, DNA-templated response to steroid hormone skeletal system morphogenesis embryonic skeletal system development response to cAMP response to hyperoxia face morphogenesis bone trabecula formation cartilage development involved in endochondral bone morphogenesis protein heterotrimerization cellular response to amino acid stimulus cellular response to mechanical stimulus cellular response to retinoic acid cellular response to vitamin E cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway response to fluoride cellular response to fluoride transforming growth factor beta receptor signaling pathway Rho protein signal transduction blood coagulation regulation of blood pressure platelet activation extracellular matrix organization collagen catabolic process odontogenesis regulation of immune response leukocyte migration cellular response to organic substance response to norepinephrine cellular response to thyroid hormone stimulus
          It has to be done as per old AB suggested Products section.
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