Invitrogen

Collagen I Monoclonal Antibody (5D8-G9)

Advanced Verification
3 Published Figures
3 References
View all (26) Collagen I antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-141

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10 - 1:100
-

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:1,000
-

ELISA (ELISA)

0.4-50 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D8-G9

Immunogen

Purified, full-length native human collagen I protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536845

Product Specific Information

MA1-141 detects collagen I from bovine, human, and porcine. This antibody will not cross react with collagen 2-11, or detect thermally denatured collagen. Western blot analysis of recombinant bovine collagen I using MA1-141 detected a predominant band around 270kD likely corresponding to the alpha-1 dimer and a fainter band at a higher MW likely corresponding to the alpha-1/alpha-2 trimer.

MA1-141 has been successfully used in ELISA, immunofluorescence, immunohistochemistry (frozen and paraffin), and Western blot procedures. The epitope is sensitive to routine formalin fixation and paraffin embedding. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain

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Gene Aliases: BOS_18945; BOS_4009; COL1A1; COL1A2; EDSC; OI1; OI2; OI3; OI4

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UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465

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Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Pig) 100626716

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Function(s)
extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding
Process(es)
skeletal system development blood vessel development transforming growth factor beta receptor signaling pathway Rho protein signal transduction blood coagulation regulation of blood pressure platelet activation extracellular matrix organization collagen fibril organization collagen catabolic process odontogenesis skin morphogenesis regulation of immune response leukocyte migration protein heterotrimerization cellular response to amino acid stimulus osteoblast differentiation intramembranous ossification endochondral ossification visual perception sensory perception of sound positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion protein transport positive regulation of cell migration response to corticosteroid response to estradiol collagen biosynthetic process protein localization to nucleus tooth mineralization response to drug response to hydrogen peroxide response to peptide hormone cellular response to fibroblast growth factor stimulus tooth eruption positive regulation of transcription, DNA-templated embryonic skeletal system development response to cAMP response to hyperoxia face morphogenesis bone trabecula formation cartilage development involved in endochondral bone morphogenesis cellular response to mechanical stimulus cellular response to retinoic acid cellular response to vitamin E cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway cellular response to fluoride
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