Invitrogen

Collagen I Monoclonal Antibody (COL-1)

4 Published Figures

23 References

View all (26) Collagen I antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (4)

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

Collagen I Antibody (MA1-26771) in IHC (P)

Collagen I Monoclonal Antibody (COL-1) detects Collagen I protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rabbit tongue. Collagen I stained by Collagen I Monoclonal Antibody (COL-1) (Product # MA1-26771) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Collagen I Antibody in Immunohistochemistry (Frozen) (IHC (F))

Collagen I Antibody in Immunocytochemistry (ICC/IF)

Collagen I Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Collagen I Antibody in Western Blot (WB)

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Product Details

MA1-26771

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:2,000

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

Dot blot (DB)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Deer, Human, Mouse, Pig, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

COL-1

Immunogen

Bovine skin collagen type I.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2081889

Product Specific Information

The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.|Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis.

International journal of molecular sciences

Kim MK,Lee JU,Lee SJ,Chang HS,Park JS,Park CS

MA1-26771 was used in Western Blot to support an inhibitory effect of EPB41L3 on the process of fibrosis and suggest the therapeutic potential of EPB41L3 as an anti-fibrotic mediator.

Thu Jun 15 00:00:00 UTC 2023

Hydroxyapatite-filled osteoinductive and piezoelectric nanofibers for bone tissue engineering.

Science and technology of advanced materials

Barbosa F,Garrudo FFF,Alberte PS,Resina L,Carvalho MS,Jain A,Marques AC,Estrany F,Rawson FJ,Aléman C,Ferreira FC,Silva JC

MA1-26771 was used in Immunohistochemistry-immunofluorescence to describe the potential of combining PVDF-TrFE and HAp for developing electroactive and osteoinductive nanofibers capable of supporting bone tissue regeneration.

Mon Sep 11 00:00:00 UTC 2023

Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.

Bioengineering & translational medicine

Yamada S,Yassin MA,Torelli F,Hansmann J,Green JBA,Schwarz T,Mustafa K

MA1-26771 was used in Immunohistochemistry to analyse the cytoskeletal modulation and osteogenic profiles of BMSC under fluid stimuli in a 3D culture condition using a perfusion bioreactor.

Mon May 01 00:00:00 UTC 2023

Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite.

Journal of materials science. Materials in medicine

Villaseñor-Cerón LS,Mendoza-Anaya D,López-Ortiz S,Rosales-Ibañez R,Rodríguez-Martínez JJ,Reyes-Valderrama MI,Rodríguez-Lugo V

MA1-26771 was used in Immunocytochemistry to study the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)2 and (NH4)2HPO4, incorporating MnCl2 to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations.

Sat Jul 29 00:00:00 UTC 2023

Lysyl oxidase-like 2 processing by factor Xa modulates its activity and substrate preference.

Communications biology

Wang H,Poe A,Martinez Yus M,Pak L,Nandakumar K,Santhanam L

MA1-26771 was used in Western Blot to show that Factor Xa (FXa) is a protease that processes LOXL2 at Arg-338.

Fri Apr 07 00:00:00 UTC 2023

Novel Electroactive Mineralized Polyacrylonitrile/PEDOT:PSS Electrospun Nanofibers for Bone Repair Applications.

International journal of molecular sciences

Barbosa F,Garrudo FFF,Marques AC,Cabral JMS,Morgado J,Ferreira FC,Silva JC

MA1-26771 was used in Immunohistochemistry-immunofluorescence to develop electroconductive polyacrylonitrile/poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PAN/PEDOT:PSS) nanofibers via electrospinning, which are capable of emulating the native tissue's fibrous extracellular matrix (ECM) and providing a platform for the delivery of exogenous electrical stimulation (ES).

Fri Aug 25 00:00:00 UTC 2023

Peripheral Nerve Decellularization for In Vitro Extracellular Matrix Hydrogel Use: A Comparative Study.

ACS biomaterials science & engineering

Gregory E,Baek IH,Ala-Kokko N,Dugan R,Pinzon-Herrera L,Almodóvar J,Song YH

MA1-26771 was used in Immunohistochemistry to establish a standard for peripheral nerve decellularization for usage as a dECM hydrogel testbed for in vitro peripheral nerve disease modeling.

Mon Jun 13 00:00:00 UTC 2022

Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model.

International journal of molecular sciences

Lee SY,Lee MH,Jo SK,Yoo IH,Sarankhuu BE,Kim HJ,Kang YE,Lee SE,Kim TY,Park MH,Lee CS,Han SY,Moon JH,Jung JY,Hong GL,Yoo NJ,Yoon ES,Choi JK,Won HR,Son JW,Song JH

MA1-26771 was used in Western Blotting to investigate the relationship between particulate matter exposure and tendon healing.

Mon Jul 25 00:00:00 UTC 2022

Fate-mapping of erythropoietin-producing cells in mouse models of hypoxaemia and renal tissue remodelling reveals repeated recruitment and persistent functionality.

Acta physiologica (Oxford, England)

Dahl SL,Pfundstein S,Hunkeler R,Dong X,Knöpfel T,Spielmann P,Scholz CC,Nolan KA,Wenger RH

MA1-26771 was used in Immunohistochemistry-immunofluorescence to test the hypothesis that REP cell migration, loss and/or differentiation may cause Epo inhibition.

Tue Mar 01 00:00:00 UTC 2022

Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis.

Biomedicines

Savin IA,Markov AV,Zenkova MA,Sen'kova AV

MA1-26771 was used in Immunohistochemistry to understand the molecular mechanisms of pulmonary fibrosis development.

Thu Apr 28 00:00:00 UTC 2022

Local accumulation of extracellular matrix regulates global morphogenetic patterning in the developing mammary gland.

Current biology : CB

Nerger BA,Jaslove JM,Elashal HE,Mao S,Košmrlj A,Link AJ,Nelson CM

MA1-26771 was used in Immunohistochemistry to reveal that local mechanical properties regulate the global pattern of mammary epithelial branching and may provide new insight into the global patterning of other branched epithelia.

Mon May 10 00:00:00 UTC 2021

An in situ activity assay for lysyl oxidases.

Communications biology

Wang H,Poe A,Pak L,Nandakumar K,Jandu S,Steppan J,Löser R,Santhanam L

MA1-26771 was used in Dot Blot, ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to develop an in situ activity assay by utilizing the strong reaction between hydrazide and aldehyde to label the LOX-catalyzed allysine (-CHO) residues with biotin-hydrazide.

Mon Jul 05 00:00:00 UTC 2021

An in situ activity assay for lysyl oxidases.

Communications biology

Wang H,Poe A,Pak L,Nandakumar K,Jandu S,Steppan J,Löser R,Santhanam L

Mon Jul 05 00:00:00 UTC 2021

An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating Cardiomyocytes.

Frontiers in cell and developmental biology

Fu W,Liao Q,Li L,Shi Y,Zeng A,Zeng C,Wang WE

Published figure using Collagen I monoclonal antibody (Product # MA1-26771) in Immunohistochemistry (PFA fixed)

Fri Oct 30 00:00:00 UTC 2020

Bioengineering the Oxygen-Deprived Tumor Microenvironment Within a Three-Dimensional Platform for Studying Tumor-Immune Interactions.

Frontiers in bioengineering and biotechnology

Bhattacharya S,Calar K,Evans C,Petrasko M,de la Puente P

MA1-26771 was used in Immunocytochemistry-immunoflourescence to study the role of oxygen availability and deprivation in tumour-immune interactions.

Sun Nov 12 00:00:00 UTC 2023

Bioengineering the Oxygen-Deprived Tumor Microenvironment Within a Three-Dimensional Platform for Studying Tumor-Immune Interactions.

Frontiers in bioengineering and biotechnology

Bhattacharya S,Calar K,Evans C,Petrasko M,de la Puente P

MA1-26771 was used in Immunocytochemistry-immunoflourescence to study the role of oxygen availability and deprivation in tumour-immune interactions.

Sun Nov 12 00:00:00 UTC 2023

Nox4 Expression Is Not Required for OVX-Induced Osteoblast Senescence and Bone Loss in Mice.

JBMR plus

Chen JR,Lazarenko OP,Zhao H,Wankhade UD,Pedersen K,Watt J,Ronis MJJ

MA1-26771 was used in Western Blotting to suggest that Nox4-mediated ROS in bone osteoblastic cells may be dispensable for sex steroid deficiency-induced bone loss and senescence in mice.

Sat Aug 01 00:00:00 UTC 2020

Nox4 Expression Is Not Required for OVX-Induced Osteoblast Senescence and Bone Loss in Mice.

JBMR plus

Chen JR,Lazarenko OP,Zhao H,Wankhade UD,Pedersen K,Watt J,Ronis MJJ

MA1-26771 was used in Western Blotting to suggest that Nox4-mediated ROS in bone osteoblastic cells may be dispensable for sex steroid deficiency-induced bone loss and senescence in mice.

Sat Aug 01 00:00:00 UTC 2020

Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome.

eLife

Bersini S,Schulte R,Huang L,Tsai H,Hetzer MW

MA1-26771 was used in Immunohistochemistry-immunofluorescence to show that iVECs and iSMCs retain disease-related signatures, allowing modeling of vascular aging and HGPS in vitro.

Tue Sep 08 00:00:00 UTC 2020

Recombinant AAV-CEA Tumor Vaccine in Combination with an Immune Adjuvant Breaks Tolerance and Provides Protective Immunity.

Molecular therapy oncolytics

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

MA1-26771 was used in ELISA to illustrate the feasibility of utilizing rAAV vectors in combination with an immunostimulatory adjuvant to break tolerance to weakly immunogenic self-antigens and for an anti-tumor response.

Fri Mar 29 00:00:00 UTC 2019

Recombinant AAV-CEA Tumor Vaccine in Combination with an Immune Adjuvant Breaks Tolerance and Provides Protective Immunity.

Molecular therapy oncolytics

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Fri Mar 29 00:00:00 UTC 2019

Three-dimensional poly lactic-co-glycolic acid scaffold containing autologous platelet-rich plasma supports keloid fibroblast growth and contributes to keloid formation in a nude mouse model.

Journal of dermatological science

Chen C,Wang H,Zhu G,Sun Z,Xu X,Li F,Luo S

MA1-26771 was used in an ELISA assay to show that the three-dimensional PLGA scaffold containing PRP supports keloid fibroblast growth and contributes to keloid formation in a nude mouse model.

Mon Jan 01 00:00:00 UTC 2018

Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model.

Stem cell research & therapy

Ebrahim N,Mostafa O,El Dosoky RE,Ahmed IA,Saad AS,Mostafa A,Sabry D,Ibrahim KA,Farid AS

MA1-26771 was used in Western Blotting to establish that the synergistic action of human UCMSCs-EVs combined with estrogen provides a highly effective alternative regenerative agent in intrauterine adhesion treatment.

Thu Jun 28 00:00:00 UTC 2018

Qualitative and quantitative assessment of collagen and elastin in annulus fibrosus of the physiologic and scoliotic intervertebral discs.

Journal of the mechanical behavior of biomedical materials

Kobielarz M,Szotek S,Głowacki M,Dawidowicz J,Pezowicz C

MA1-26771 was used in ELISA to study annulus fibrosus of the physiologic and scoliotic intervetebral discs by a qualitiative and quantitative assessment of elastin and collagen

Thu Sep 01 00:00:00 UTC 2016

Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism.

Infection and immunity

Sémiramoth N,Gleizes A,Turbica I,Sandré C,Marin-Esteban V,Gorges R,Servin A,Chollet-Martin S

MA1-26771 was used in western blot to study the mechanism for enterovirulence of Escherichia coli strain C1845

Thu Jul 01 00:00:00 UTC 2010

Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.

Journal of immunology (Baltimore, Md. : 1950)

Cusi MG,Del Vecchio MT,Terrosi C,Savellini GG,Di Genova G,La Placa M,Fallarino F,Moser C,Cardone C,Giorgi G,Francini G,Correale P

MA1-26771 was used in immunohistochemistry to investigate the influence of CD40L on the efficiency of specific anticancer vaccine

Wed Jun 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: alpha 1 type I collagen; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

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Gene Aliases: AA960264; AI325291; BOS_18945; BOS_4009; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

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UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

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Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Rabbit) 100008997, (Human) 1278, (Bovine) 282188, (Pig) 100626716, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352, (Rabbit) 100347598

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Function(s)

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding molecular_function

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Collagen I Monoclonal Antibody (COL-1)

Collagen I Antibody (MA1-26771) in IHC (P)

Product Details

MA1-26771

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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