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Collagen Type II Antibody (600-401-104-01) in IHC
Rockland anti collagen II antibody (600-401-104 Lot 26014, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, ... View More
Product Details
600-401-104-01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Sheep,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Collagen Type II from human knee cartilage and bovine nasal cartilage.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
ypically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; cyanogen bromide; disproportionate micromelia; MGC131516; pro-alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1
Gene Aliases: ANFH; AOM; BOS_5148; CB11; CG2A1A; COL11A3; Col2; Col2a; Col2a-1; COL2A1; COLLII; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1
UniProt ID: (Human) P02458, (Bovine) P02459, (Rat) P05539, (Mouse) P28481
Entrez Gene ID: (Human) 1280, (Sheep) 100196905, (Bovine) 407142, (Rat) 25412, (Mouse) 12824
skeletal system development
cartilage condensation
tissue homeostasis
endochondral ossification
chondrocyte differentiation
heart morphogenesis
growth plate cartilage development
proteoglycan metabolic process
central nervous system development
visual perception
sensory perception of sound
response to mechanical stimulus
response to X-ray
regulation of gene expression
collagen fibril organization
notochord development
limb morphogenesis
inner ear morphogenesis
skeletal system morphogenesis
inner ear development
cartilage development
palate development
limb bud formation
embryonic skeletal joint morphogenesis
bone development
cartilage development involved in endochondral bone morphogenesis
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to nicotine
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
cellular response to peptide hormone stimulus
otic vesicle development
cellular response to BMP stimulus
response to fibroblast growth factor
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
extracellular matrix organization
collagen catabolic process
regulation of immune response
negative regulation of apoptotic process
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