Invitrogen

Collagen X Monoclonal Antibody (X53), eFluor™ 570, eBioscience™

9 Published Figures

11 References

View all (9) Collagen X antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

Collagen X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

Collagen X Antibody (41-9771-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 5 µg/mL of Mouse IgG1 K Isotype Control eFluor® 570 (left) or 5 µg/mL of Anti-Collagen Type X eFluor® 570 (right). Nuclei are stained with DAPI.

Collagen X Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Collagen X Antibody in Immunohistochemistry (IHC)

Collagen X Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

Collagen X Antibody in Western Blot (WB)

Product Image Gallery

Product Details

41-9771-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Pig, Rat, Dog, Guinea pig, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 570, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

X53

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573641

Product Specific Information

Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X. X53 does not cross-react with collagen types I, II, II, IV, or VI. Collagen Type X is a homotrimeric short chain collagen. Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development. Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization. Collagen Type X is elevated in many tumor types with expression being localized in the vasculature. Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia.

Applications Reported: This X53 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tuning the Phenotype of Cartilage Tissue Mimics by Varying Spheroid Maturation and Methacrylamide-Modified Gelatin Hydrogel Characteristics.

Macromolecular bioscience

De Moor L,Minne M,Tytgat L,Vercruysse C,Dubruel P,Van Vlierberghe S,Declercq H

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Sat May 01 00:00:00 UTC 2021

Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development.

Journal of cellular physiology

So EY,Sun C,Wu KQ,Driesman A,Leggett S,Isaac M,Spangler T,Dubielecka-Szczerba PM,Reginato AM,Liang OD

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Sat Feb 01 00:00:00 UTC 2020

Angiogenic Potential of Tissue Engineered Cartilage From Human Mesenchymal Stem Cells Is Modulated by Indian Hedgehog and Serpin E1.

Frontiers in bioengineering and biotechnology

Nossin Y,Farrell E,Koevoet WJLM,Somoza RA,Caplan AI,Brachvogel B,van Osch GJVM

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Thu Dec 31 00:00:00 UTC 2020

Promoting Effect of Basic Fibroblast Growth Factor in Synovial Mesenchymal Stem Cell-Based Cartilage Regeneration.

International journal of molecular sciences

Okamura G,Ebina K,Hirao M,Chijimatsu R,Yonetani Y,Etani Y,Miyama A,Takami K,Goshima A,Yoshikawa H,Ishimoto T,Nakano T,Hamada M,Kanamoto T,Nakata K

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Wed Dec 30 00:00:00 UTC 2020

Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head.

International journal of molecular sciences

Herrmann M,Hildebrand M,Menzel U,Fahy N,Alini M,Lang S,Benneker L,Verrier S,Stoddart MJ,Bara JJ

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Sun Jul 14 00:00:00 UTC 2019

Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion.

Scientific reports

Murahashi Y,Yano F,Chijimatsu R,Nakamoto H,Maenohara Y,Amakawa M,Miyake Y,Yamanaka H,Iba K,Yamashita T,Tanaka S,Saito T

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry (PFA fixed)

Mon Dec 30 00:00:00 UTC 2019

Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion.

Scientific reports

Murahashi Y,Yano F,Chijimatsu R,Nakamoto H,Maenohara Y,Amakawa M,Miyake Y,Yamanaka H,Iba K,Yamashita T,Tanaka S,Saito T

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry (PFA fixed)

Mon Dec 30 00:00:00 UTC 2019

A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering.

Materials (Basel, Switzerland)

Bartnikowski M,Akkineni AR,Gelinsky M,Woodruff MA,Klein TJ

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunofluorescence

Thu Apr 14 00:00:00 UTC 2016

A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering.

Materials (Basel, Switzerland)

Bartnikowski M,Akkineni AR,Gelinsky M,Woodruff MA,Klein TJ

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunofluorescence

Thu Apr 14 00:00:00 UTC 2016

The gonadotrophin-releasing hormone receptor of alpha T3-1 pituitary cells regulates cellular levels of both of the phosphoinositidase C-linked G proteins, Gq alpha and G11 alpha, equally.

Molecular pharmacology

Shah BH,Milligan G

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Fri Jul 01 00:00:00 UTC 1994

The gonadotrophin-releasing hormone receptor of alpha T3-1 pituitary cells regulates cellular levels of both of the phosphoinositidase C-linked G proteins, Gq alpha and G11 alpha, equally.

Molecular pharmacology

Shah BH,Milligan G

Published figure using Collagen X monoclonal antibody (Product # 41-9771-82) in Immunohistochemistry

Fri Jul 01 00:00:00 UTC 1994

Bioinformatics

Protein Aliases: Collagen alpha-1(X) chain; collagen type X alpha 1; collagen X, alpha-1 polypeptide; collagen, type X, alpha 1; collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia); pro alpha 1; procollagen, type X, alpha 1; Schmid metaphyseal chondrodysplasia; type X collagen; type X collagen, alpha 1

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Gene Aliases: Col10; Col10a-1; COL10A1

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UniProt ID: (Human) Q03692, (Mouse) Q05306

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Entrez Gene ID: (Pig) 448809, (Human) 1300, (Dog) 100856711, (Mouse) 12813, (Rat) 25681

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Function(s)

protein binding metal ion binding molecular_function extracellular matrix structural constituent

Process(es)

endochondral ossification cartilage development skeletal system development extracellular matrix organization collagen catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Collagen X Monoclonal Antibody (X53), eFluor™ 570, eBioscience™

Collagen X Antibody (41-9771-82) in IHC (P)

Product Details

41-9771-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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