Invitrogen

Complement C3 Polyclonal Antibody

Complement C3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Complement C3 Antibody (PA1-29715) in ICC/IF

ASN neuro 2022 - Figure 7. Assessment of the functional state of reactive astrocytes after TMT exposure. Double immunofluorescent staining of GFAP and IL-1beta, TNF-alpha, IL-10, C3, iNOS and NF-kB and corresponding integrated fluorescence density expressed as AUs +- SEM in the injured CA area at 7 and 21 dpi. Significance shown inside the graphs: * p < .05 or less compared to age-match Ctrl. Note . AU = arbitrary unit;... View More

Complement C3 Antibody in Immunohistochemistry (IHC)

Complement C3 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

PA1-29715

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100

Immunoelectrophoresis (IE)

Assay-dependent

Product Specifications

Species Reactivity

Baboon, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Cynomolgus monkey, Guinea pig, Human, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human complement C3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

TBS, pH 7.5, with 500mM NaCl

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2066730

Product Specific Information

Specificity: Monospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b and C3c.

Product is supplied as diluted unpurified antiserum. Store as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantifying systemic molecular networks affected during high altitude de-acclimatization.

Scientific reports

Paul S,Jain S,Gangwar A,Mohanty S,Khan N,Ahmad Y

PA1-29715 was used in Western Blotting to provide a conceptual framework for formulating therapeutic strategies to ease the symptoms of HADAS during de-acclimatization.

Thu Sep 07 00:00:00 UTC 2023

Quantifying systemic molecular networks affected during high altitude de-acclimatization.

Scientific reports

Paul S,Jain S,Gangwar A,Mohanty S,Khan N,Ahmad Y

PA1-29715 was used in Western Blotting to provide a conceptual framework for formulating therapeutic strategies to ease the symptoms of HADAS during de-acclimatization.

Thu Sep 07 00:00:00 UTC 2023

A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability.

Scientific reports

AlQot HE,Rylett RJ

PA1-29715 was used in Western Blotting to establish a novel transgenic mouse expressing 82-kDa ChAT that is valuable for studying the role of this primate-specific cholinergic enzyme in pathologies associated with cholinergic neuron vulnerability and dysfunction.

Tue Feb 21 00:00:00 UTC 2023

Complement C4-deficient mice have a high mortality rate during PTZ-induced epileptic seizures, which correlates with cognitive problems and the deficiency in the expression of Egr1 and other immediate early genes.

Frontiers in cellular neuroscience

Veremeyko T,Jiang R,He M,Ponomarev ED

PA1-29715 was used in Western Blotting to demonstrate a new role of C4B in the regulation of expression of IEGs and their downstream targets during CNS insults such as epileptic seizures.

Sun May 28 00:00:00 UTC 2023

Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration.

ASN neuro

Dragić M,Mitrović N,Adžić M,Nedeljković N,Grković I

PA1-29715 was used in Immunocytochemistry, Immunohistochemistry to investigated spatial and temporal patterns of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase1/CD39) and ecto-5' nucleotidase (eN/CD73) activity, their cell-specific localization, and analyzed gene expression pattern and/or cellular localization of purinoreceptors and proinflammatory mediators associated with reactive glial cells.

Mon Jan 17 00:00:00 UTC 2022

Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration.

ASN neuro

Dragić M,Mitrović N,Adžić M,Nedeljković N,Grković I

Mon Jan 17 00:00:00 UTC 2022

Prolonged intraocular residence and retinal tissue distribution of a fourth-generation compstatin-based C3 inhibitor in non-human primates.

Clinical immunology (Orlando, Fla.)

Hughes S,Gumas J,Lee R,Rumano M,Berger N,Gautam AK,Sfyroera G,Chan AL,Gnanaguru G,Connor KM,Kim BJ,Dunaief JL,Ricklin D,Hajishengallis G,Yancopoulou D,Reis ES,Mastellos DC,Lambris JD

PA1-29715 was used in Immunohistochemistry to report the intraocular distribution and pharmacokinetic profile of the fourth-generation compstatin analog, Cp40-KKK in cynomolgus monkeys following a single intravitreal injection.

Fri May 01 00:00:00 UTC 2020

Podocytes Produce and Secrete Functional Complement C3 and Complement Factor H.

Frontiers in immunology

Mühlig AK,Keir LS,Abt JC,Heidelbach HS,Horton R,Welsh GI,Meyer-Schwesinger C,Licht C,Coward RJ,Fester L,Saleem MA,Oh J

Published figure using Complement C3 polyclonal antibody (Product # PA1-29715) in Western Blot

Mon Apr 12 00:00:00 UTC 2021

Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.

Frontiers in neuroscience

Jakovljevic M,Lavrnja I,Bozic I,Milosevic A,Bjelobaba I,Savic D,Sévigny J,Pekovic S,Nedeljkovic N,Laketa D

PA1-29715 was used in Immunohistochemistry to explore the expression of ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) in the spinal cord, at the onset (Eo), peak (Ep), and end (Ee) of experimental autoimmune encephalomyelitis (EAE).

Thu Oct 01 00:00:00 UTC 2020

Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.

Frontiers in neuroscience

Jakovljevic M,Lavrnja I,Bozic I,Milosevic A,Bjelobaba I,Savic D,Sévigny J,Pekovic S,Nedeljkovic N,Laketa D

Thu Oct 01 00:00:00 UTC 2020

The immunosuppression mechanism of hypodermin A on complement.

Parasitology international

Chen RJ,Hu AK,Yuan HH,Wang ZZ,Ji DJ,Wu LL,Zhang TY,Zhu YH,Sun W,Zhu XR

PA1-29715 was used in western blot to study the mechanism by which hypodermin A inhibits the complement pathway

Tue Apr 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement C3 alpha chain; complement component 3; complement component C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3

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Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; C3d; CPAMD1; HEL-S-62p; HSE-MSF; I79_008227; Plp

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UniProt ID: (Guinea pig) P12387, (Rat) P01026, (Human) P01024, (Mouse) P01027, (Pig) P01025

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Entrez Gene ID: (Guinea pig) 100135462, (Chinese hamster) 100763958, (Rat) 24232, (Rabbit) 100009121, (Human) 718, (Mouse) 12266, (Pig) 397072

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Function(s)

endopeptidase inhibitor activity protein binding lipid binding C5L2 anaphylatoxin chemotactic receptor binding cofactor binding serine-type endopeptidase activity receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Complement C3 Polyclonal Antibody

Complement C3 Antibody (PA1-29715) in ICC/IF

Product Details

PA1-29715

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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