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Complement C3 Antibody (PA1-40288) in WB
Western blot was performed using Anti-Complement C3 Polyclonal Antibody (Product # PA1-40288) and a 187 kDa band corresponding to Complement C3 was observed in Hep G2 with LPS and PTI treatment. A non specific band around 95 kDa was also observed. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein transport inhibitor (1X for 4hr) (Lane 2) and Hep G2 treated with LPS (10 ng/mL for 18hr) and Protein transport inhibitor (1X for 4 hr) (Lane 3) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Pro... View More
Product Details
PA1-40288
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Murine C3.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2274957
Product Specific Information
PA1-40288 detects C3 from mouse samples.
PA1-40288 has been successfully used in immunohistochemistry (frozen), immunohistochemistry (paraffin), immunofluorescence and Western blot applications. The antibody works well in both PBS and in TBS.
The PA1-40288 imunogen is mouse C3.
Positive control: Kidney, Spleen.
Target Information
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3
Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp
UniProt ID: (Human) P01024, (Mouse) P01027
Entrez Gene ID: (Human) 718, (Mouse) 12266
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
proteolysis
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
regulation of complement activation
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
regulation of immune response
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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