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Invitrogen
Complement C3b Monoclonal Antibody (10C7)
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FIGURE: 1 / 4
Complement C3b Antibody (MA1-70054) in Flow
Flow cytometric analysis of C57BL/6 mouse thymocytes incubated with rabbit anti-mouse T cells and then incubated with fresh human serum were stained using a Complement C3b monoclonal antibody (Product # MA1-70054) (filled histogram) or mouse IgG1 isotype control (open histogram).
Product Details
MA1-70054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10C7
Immunogen
C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C3).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1073822
Product Specific Information
MA1-70054 detects C3/C3b/iC3b in human and mouse samples.
MA1-70054 has been successfully used in flow cytometry and ELISA procedures.
The MA1-70054 immunogen is C57BL/6 thymocytes incubated with a rat IgG2b anti-mouse Thy-1 (RmT1) and C57BL/6 serum (as a source for C3).
Target Information
Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; iC3b; prepro-C3
Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp
UniProt ID: (Human) P01024, (Mouse) P01027
Entrez Gene ID: (Human) 718, (Mouse) 12266
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
proteolysis
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
regulation of complement activation
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
regulation of immune response
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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