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Invitrogen
Complement Factor I Polyclonal Antibody
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FIGURE: 1 / 2
Complement Factor I Antibody (PA5-96371) in WB
Western blot analysis of Complement Factor I in extracts of Human serum. Samples were incubated with Complement Factor I Polyclonal antibody (Product # PA5-96371) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-96371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human CFI (NP_0001952)
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808173
Product Specific Information
Immunogen sequence: KVTYTSQEDL VEKKCLAKKY THLSCDKVFC QPWQRCIEGT CVCKLPYQCP KNGTAVCATN RRSFPTYCQQ KSLECLHPGT KFLNNGTCTA EGKFSVSLKH GNTDSEGIVE VKLVDQDKTM FICKSSWSMR EANVACLDLG FQQGADTQRR FKLSDLSINS TECLHVHCRG LETSLAECTF TKRRTMGYQD FADVVCYTQK ADSPMDDFFQ CVNGKYISQM KACDGINDCG DQSDELCCKA CQGKGFHCKS GVCIPSQYQC NGEVDCITGE DEVGCAGFAS VA; Positive Samples: Mouse liver, Mouse intestine; Cellular Location: extracellular space
Target Information
Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88 kDa, processed to yield the active form, composed of two disulfide-linked chains, the a-chain (50 kDa) and the b-chain (38 kDa). Plasma concentration is 35 µg/mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C3b-inactivator; C3B/C4B inactivator; complement component I; complement control protein factor I; Complement factor I; complement factor I heavy chain; factor i; Konglutinogen-activating factor; light chain of factor I
Gene Aliases: AHUS3; ARMD13; C3b-INA; C3BINA; CFI; FI; IF; KAF
UniProt ID: (Human) P05156, (Mouse) Q61129
Entrez Gene ID: (Human) 3426, (Mouse) 12630
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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