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Invitrogen
Connexin 30 Recombinant Rabbit Monoclonal Antibody (16H9L8)
FIGURE: 1 / 11
Connexin 30 Antibody (700258) in ICC/IF
Immunofluorescent analysis of Connexin 30 was performed on 90% confluent log phase Caco-2 cells The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 30 Recombinant Rabbit Monoclonal Antibody (Product # 700258) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled wi... View More
Product Details
700258
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16H9L8
Immunogen
A peptide corresponding to amino acids 241-261 of P70689.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532309
Product Specific Information
This antibody is predicted to react with human, rat, hamster and bovine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 30; Connexin-30; Cx30; ectodermal dysplasia 2, hidrotic (Clouston syndrome); Gap junction beta-6 protein; gap junction membrane channel protein beta 6; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6, 30kDa
Gene Aliases: AA958971; CX30; Cxn-30; D14Bwg0506e; DFNA3; DFNA3B; DFNB1B; ECTD2; ED2; EDH; GJB6; HED; HED2
UniProt ID: (Human) O95452, (Mouse) P70689
Entrez Gene ID: (Human) 10804, (Mouse) 14623
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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