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Invitrogen
Connexin 31 Polyclonal Antibody
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FIGURE: 1 / 6
Connexin 31 Antibody (36-5100) in ICC/IF
Immunofluorescence analysis of CONNEXIN 31 was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Connexin 31 Rabbit Polyclonal Antibody (Product # 36-5100) at 2µg/mL in 0.1% BSA and incubated for 3 hours a... View More
Product Details
36-5100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of mouse connexin 31 protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533265
Product Specific Information
This antibody reacts with the ~32 kDa mouse Connexin 31 on Western blots; the band observed at ~62 kDa is probably due to dimerization of Connexin 31. Several higher bands of unknown origin are observed in western blots of mouse skin homogenates.
Target Information
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 31; Connexin-31; Cx31; Gap junction beta-3 protein; gap junction membrane channel protein beta 3; gap junction protein, beta 3, 31kDa
Gene Aliases: Cnx31; CX31; Cxn-31; D4Wsu144e; DFNA2; DFNA2B; EKV; Gjb-3; GJB3
UniProt ID: (Human) O75712, (Mouse) P28231
Entrez Gene ID: (Human) 2707, (Mouse) 14620
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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