Invitrogen

Connexin 40 Polyclonal Antibody

Connexin 40 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Connexin 40 Antibody (36-4900) in IHC (P)

Immunohistochemistry analysis of Connexin 40 showing staining in the membrane of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature... View More

Connexin 40 Antibody in Western Blot (WB)

Connexin 40 Antibody in Immunoprecipitation (IP)

Connexin 40 Antibody in Immunocytochemistry (ICC/IF)

Connexin 40 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Connexin 40 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Connexin 40 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

36-4900

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:50

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse and rat Connexin 40 (Cx40) proteins. The human sequence at this location differs by only one amino acid.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533263

Target Information

Connexin 40 (Cx40), belongs to the connexin family of gap junction proteins, which are expressed in pancreas, kidney, skeletal muscle, liver, placenta and heart. Connexin 40 is highly expressed in vascular endothelial cells, and the mammalian heart (specifically in the atrial myocardium, and the His-Purkinje conduction system). At least three other connexins are expressed in the heart, including Cx37 (endocardium), Cx43 (atrial and ventricular myocardium, and distal parts of the conduction system) and Cx45 (throughout the heart). Cx40 and Cx43 frequently co-localize in cells of the cardiovascular system and participate in the formation of heterotypic gap junction channels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Involvement of Cx43 in JNK1/2-Mediated Endothelial Mechanotransduction and Human Plaque Progression.

International journal of molecular sciences

Tauchi M,Oshita K,Urschel K,Furtmair R,Kühn C,Stumpfe FM,Botos B,Achenbach S,Dietel B

36-4900 was used in Immunocytochemistry to investigate SS-induced endothelial activation, focusing on stress-regulated mitogen-activated protein kinases (MAPK) and downstream signaling, and its relation to gap junction proteins, Connexins (Cxs).

Sat Jan 07 00:00:00 UTC 2023

The Involvement of Cx43 in JNK1/2-Mediated Endothelial Mechanotransduction and Human Plaque Progression.

International journal of molecular sciences

Tauchi M,Oshita K,Urschel K,Furtmair R,Kühn C,Stumpfe FM,Botos B,Achenbach S,Dietel B

Sat Jan 07 00:00:00 UTC 2023

K_IR channel regulation of electrical conduction along cerebrovascular endothelium: Enhanced modulation during Alzheimer's disease.

Microcirculation (New York, N.Y. : 1994)

Hakim MA,Behringer EJ

36-4900 was used in Immunohistochemistry to addressed effects of inward-rectifying K+ (KIR ) channel activity on endothelial cell coupling before and during Alzheimer's disease (AD).

Sun Jan 01 00:00:00 UTC 2023

Molecular mechanisms of postoperative atrial fibrillation in patients with obstructive sleep apnea.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

López-Gálvez R,Rivera-Caravaca JM,Mandaglio-Collados D,Orenes-Piñero E,Lahoz Á,Hernández-Romero D,Martínez CM,Carpes M,Arribas JM,Cánovas S,Lip GYH,Marín F

36-4900 was used in Immunocytochemistry to investigate the pathophysiological mechanisms of AF in association with OSA in a cohort of cardiac surgery patients.

Thu Jun 01 00:00:00 UTC 2023

Endocardial role in arrhythmias induced by acute ventricular stretch and the involvement of Purkinje fibres, in isolated rat hearts.

Current research in physiology

Hurley M,Kaur S,Walton R,Power A,Haïssaguerre M,Bernus O,Ward ML,White E

36-4900 was used in Immunohistochemistry to show that the endocardial surface plays an important role in these mechanically-induced ectopic activations.

Fri Feb 24 00:00:00 UTC 2023

TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.

International journal of biological sciences

Qu K,Wang C,Huang L,Qin X,Zhang K,Zhong Y,Ma Q,Yan W,Li T,Peng Q,Wang Y,Gregersen H,Tang C,Qiu J,Wang G

Published figure using Connexin 40 polyclonal antibody (Product # 36-4900) in Western Blot

Fri Apr 15 00:00:00 UTC 2022

FOXO1 represses sprouty 2 and sprouty 4 expression to promote arterial specification and vascular remodeling in the mouse yolk sac.

Development (Cambridge, England)

Li-Villarreal N,Wong RLY,Garcia MD,Udan RS,Poché RA,Rasmussen TL,Rhyner AM,Wythe JD,Dickinson ME

36-4900 was used in Immunohistochemistry-immunofluorescence to demonstrate that at E8.25, loss of Foxo1 in Tie2-cre expressing cells resulted in increased sprouty 2 (Spry2) and Spry4 expression, reduced arterial gene expression and reduced Kdr transcripts without affecting overall endothelial cell identity, survival or proliferation.

Fri Apr 01 00:00:00 UTC 2022

Role of atrial arrhythmia and ventricular response in atrial fibrillation induced atrial remodelling.

Cardiovascular research

Guichard JB,Xiong F,Qi XY,L'Heureux N,Hiram R,Xiao J,Naud P,Tardif JC,Da Costa A,Nattel S

36-4900 was used in Western Blotting to assess the roles of atrial arrhythmia versus high ventricular rate in AF-associated remodeling.

Thu Jan 21 00:00:00 UTC 2021

Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22-5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication.

International journal of molecular sciences

Garcia-Elias A,Tajes M,Yañez-Bisbe L,Enjuanes C,Comín-Colet J,Serra SA,Fernández-Fernández JM,Aguilar-Agon KW,Reilly S,Martí-Almor J,Benito B

36-4900 was used in Western Blot to show that circulating miR-199a-5p and miR-22-5p are higher in heart failure with reduced ejection fraction patients with atrial fibrillation (AF), and that cells exposed to both miRNAs exhibited altered Ca2+ handling and defective cell-to-cell communication.

Sun Sep 26 00:00:00 UTC 2021

Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22-5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication.

International journal of molecular sciences

Garcia-Elias A,Tajes M,Yañez-Bisbe L,Enjuanes C,Comín-Colet J,Serra SA,Fernández-Fernández JM,Aguilar-Agon KW,Reilly S,Martí-Almor J,Benito B

Sun Sep 26 00:00:00 UTC 2021

Systemic therapy in an RNA toxicity mouse model with an antisense oligonucleotide therapy targeting a non-CUG sequence within the DMPK 3'UTR RNA.

Human molecular genetics

Yadava RS,Yu Q,Mandal M,Rigo F,Bennett CF,Mahadevan MS

36-4900 was used in Immunohistochemistry-immunofluorescence to demonstrate systemic treatment with an antisense oligonucleotide (ASO) (ISIS 486178) targeted to a non-CUG sequence within the 3'UTR of dystrophia myotonica protein kinase.

Wed Jun 03 00:00:00 UTC 2020

Importance of endothelial Hey1 expression for thoracic great vessel development and its distal enhancer for Notch-dependent endothelial transcription.

The Journal of biological chemistry

Watanabe Y,Seya D,Ihara D,Ishii S,Uemoto T,Kubo A,Arai Y,Isomoto Y,Nakano A,Abe T,Shigeta M,Kawamura T,Saito Y,Ogura T,Nakagawa O

Published figure using Connexin 40 polyclonal antibody (Product # 36-4900) in Immunohistochemistry

Fri Dec 18 00:00:00 UTC 2020

Vascular endothelial growth factor promotes atrial arrhythmias by inducing acute intercalated disk remodeling.

Scientific reports

Mezache L,Struckman HL,Greer-Short A,Baine S,Györke S,Radwański PB,Hund TJ,Veeraraghavan R

36-4900 was used in Western Blotting to show VEGF can slow atrial conduction and predispose normal hearts to atrial arrhythmias.

Tue Nov 24 00:00:00 UTC 2020

Vascular endothelial growth factor promotes atrial arrhythmias by inducing acute intercalated disk remodeling.

Scientific reports

Mezache L,Struckman HL,Greer-Short A,Baine S,Györke S,Radwański PB,Hund TJ,Veeraraghavan R

36-4900 was used in Western Blotting to show VEGF can slow atrial conduction and predispose normal hearts to atrial arrhythmias.

Tue Nov 24 00:00:00 UTC 2020

An N-/L-type calcium channel blocker, cilnidipine, suppresses autonomic, electrical, and structural remodelling associated with atrial fibrillation.

Cardiovascular research

Tajiri K,Guichard JB,Qi X,Xiong F,Naud P,Tardif JC,Costa AD,Aonuma K,Nattel S

36-4900 was used in Western Blotting to investigate the effects of Cilnidipine, a commercially available drug with NTCC-blocking action, on atrial fibrillation-related remodelling.

Sun Dec 01 00:00:00 UTC 2019

Implantation of engineered conduction tissue in the rat heart.

Molecular medicine reports

Zhang W,Li X,Sun S,Zhang X

36-4900 was used in Immunohistochemistry-immunofluorescence to determine whether engineered conduction tissue could act as an atrioventricular conduction pathway.

Mon Apr 01 00:00:00 UTC 2019

Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells.

American journal of physiology. Cell physiology

Guo R,Si R,Scott BT,Makino A

364900 was used in immunocytochemistry to determine the role of mitochondrial Cx40 in endothelial cells

Sat Apr 01 00:00:00 UTC 2017

Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification.

Nature communications

Fang JS,Coon BG,Gillis N,Chen Z,Qiu J,Chittenden TW,Burt JM,Schwartz MA,Hirschi KK

36-4900 was used in Immunocytochemistry to elucidate a mechanochemical pathway where arterial shear activates a NOTCH-GJA4-CDKN1B axis promoting endothelial cell cycle arrest to enable arterial gene expression.

Fri Dec 15 00:00:00 UTC 2017

Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins.

BMC cell biology

Gemel J,Su Z,Gileles-Hillel A,Khalyfa A,Gozal D,Beyer EC

36-4900 was used in immunohistochemistry and western blot to examine connexin40 and connexin43 expression in mouse models of hypoxia and sleep apnea

Tue Jan 17 00:00:00 UTC 2017

Specificity of the connexin W3/4 locus for functional gap junction formation.

Channels (Austin, Tex.)

Xu Q,Lin X,Matiukas A,Zhang X,Veenstra RD

36-4900 was used in Immunocytochemistry to indicate an absolute requirement for an amphipathic W3/4 residue that is conserved among all α/Β/δ/γ-group connexins.

Tue Nov 01 00:00:00 UTC 2016

Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes.

Nature communications

Eng G,Lee BW,Protas L,Gagliardi M,Brown K,Kass RS,Keller G,Robinson RB,Vunjak-Novakovic G

36-4900 was used in immunocytochemistry to determine the autonomouse beating rate adaptation in cardiomyocytes from human stem cells

Tue Jan 19 00:00:00 UTC 2016

MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator.

Nitric oxide : biology and chemistry

Kundu S,Pushpakumar S,Sen U

36-4900 was used in western blot to test if H2S supplementation reduces MMP-9-induced extracellular matrix remodeling and dysfunction in diabetic kidney.

Thu Apr 30 00:00:00 UTC 2015

Connexin40 abnormalities and atrial fibrillation in the human heart.

Journal of molecular and cellular cardiology

Gemel J,Levy AE,Simon AR,Bennett KB,Ai X,Akhter S,Beyer EC

Sat Nov 01 00:00:00 UTC 2014

Connexin40 abnormalities and atrial fibrillation in the human heart.

Journal of molecular and cellular cardiology

Gemel J,Levy AE,Simon AR,Bennett KB,Ai X,Akhter S,Beyer EC

Sat Nov 01 00:00:00 UTC 2014

Degradation of a connexin40 mutant linked to atrial fibrillation is accelerated.

Journal of molecular and cellular cardiology

Gemel J,Simon AR,Patel D,Xu Q,Matiukas A,Veenstra RD,Beyer EC

36-4900 was used in western blot to study Cx40 mutants and determine how they contribute to atrial fibrillation pathogenesis

Mon Sep 01 00:00:00 UTC 2014

Degradation of a connexin40 mutant linked to atrial fibrillation is accelerated.

Journal of molecular and cellular cardiology

Gemel J,Simon AR,Patel D,Xu Q,Matiukas A,Veenstra RD,Beyer EC

36-4900 was used in western blot to study Cx40 mutants and determine how they contribute to atrial fibrillation pathogenesis

Mon Sep 01 00:00:00 UTC 2014

Degradation of a connexin40 mutant linked to atrial fibrillation is accelerated.

Journal of molecular and cellular cardiology

Gemel J,Simon AR,Patel D,Xu Q,Matiukas A,Veenstra RD,Beyer EC

36-4900 was used in western blot to study Cx40 mutants and determine how they contribute to atrial fibrillation pathogenesis

Mon Sep 01 00:00:00 UTC 2014

Functional formation of heterotypic gap junction channels by connexins-40 and -43.

Channels (Austin, Tex.)

Lin X,Xu Q,Veenstra RD

36-4900 was used in Western Blotting to examine the formation and biophysical properties of heterotypic Connexin43/Connexin40 gap junction channels.

Tue Sep 29 00:00:00 UTC 2015

Functional formation of heterotypic gap junction channels by connexins-40 and -43.

Channels (Austin, Tex.)

Lin X,Xu Q,Veenstra RD

36-4900 was used in Western Blotting to examine the formation and biophysical properties of heterotypic Connexin43/Connexin40 gap junction channels.

Tue Sep 29 00:00:00 UTC 2015

Autosomal recessive GJA1 (Cx43) gene mutations cause oculodentodigital dysplasia by distinct mechanisms.

Journal of cell science