Invitrogen

Connexin 45 Polyclonal Antibody

Connexin 45 Antibody in Immunocytochemistry (ICC/IF)

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Connexin 45 Antibody (40-7000) in ICC/IF

Brain structure & function 2013 - Fig. 4 a , b IB4 staining is clearly observed within laminae I/II of the DH and makes close appositions to both Cx45-eGFP cells within laminae I/II and those in lamina III. c - e Confocal scan of representative cells (from lamina III in a ) demonstrate the density of projections surrounding the Cx45-eGFP cells. f , g A reconstructed model example in two orientations illustrating the density of projections o... View More

Connexin 45 Antibody in Immunohistochemistry (IHC)

Connexin 45 Antibody in Western Blot (WB)

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Product Details

40-7000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse, rat, Chinese hamster, and golden hamster connexin 45 (Cx45, GJA7, gap junction alpha-7) protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533478

Target Information

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A potential role for neuronal connexin 36 in the pathogenesis of amyotrophic lateral sclerosis.

Neuroscience letters

Belousov AB,Nishimune H,Denisova JV,Fontes JD

40-7000 was used in Western Blotting to show that the expression of Cx36 is significantly decreased in lumbar segments of the spinal cord of both human amyotrophic lateral sclerosis subjects and SOD1G93A mice as compared to healthy human and wild-type mouse controls, respectively.

Wed Feb 14 00:00:00 UTC 2018

LKB1 deletion causes early changes in atrial channel expression and electrophysiology prior to atrial fibrillation.

Cardiovascular research

Kim GE,Ross JL,Xie C,Su KN,Zaha VG,Wu X,Palmeri M,Ashraf M,Akar JG,Russell KS,Akar FG,Young LH

40-7000 was used in Western Blotting to investigate the effects of LKB1 deletion on atrial channel expression and electrophysiological function in a cardiomyocyte-specific knockout mouse model.

Thu Oct 01 00:00:00 UTC 2015

Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes.

PloS one

Noritake K,Aki T,Funakoshi T,Unuma K,Uemura K

40-7000 was used in western blot to investigate junctional intercellular communication, mechanotransduction through cytoskeletal organization, and the hippo-YAP pathway in cardiac damage caused by direct exposure to ethanol.

Tue May 24 00:00:00 UTC 2016

AII amacrine cells discriminate between heterocellular and homocellular locations when assembling connexin36-containing gap junctions.

Journal of cell science

Meyer A,Hilgen G,Dorgau B,Sammler EM,Weiler R,Monyer H,Dedek K,Hormuzdi SG

40-7000 was used in immunohistochemistry - frozen section to distinguish the difference between homocellular and heterocellular locations when amacrine cells assemble connexin36 -containing gap junctions

Sat Mar 15 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

40-7000 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus

Sat Mar 01 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

40-7000 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus

Sat Mar 01 00:00:00 UTC 2014

NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions.

Cell communication and signaling : CCS

Pogoda K,Füller M,Pohl U,Kameritsch P

40-7000 was used in immunohistochemistry to study the effect of NO on the gap junctional calcium signal propagation in endothelial cells and determine the distribution of the vascular connexins in small resistance arteries.

Thu May 15 00:00:00 UTC 2014

Gap junction proteins in the light-damaged albino rat.

Molecular vision

Guo CX,Tran H,Green CR,Danesh-Meyer HV,Acosta ML

40-7000 was used in immunohistochemistry - frozen section to research light-damaged albino rats and their gap junction proteins

Tue Sep 30 00:00:00 UTC 2014

Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice.

Brain structure & function

Chapman RJ,Lall VK,Maxeiner S,Willecke K,Deuchars J,King AE

40-7000 was used in immunohistochemistry to identify Cx45-positive neurones in the adult spinal dorsal horn

Wed May 01 00:00:00 UTC 2013

Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice.

Brain structure & function

Chapman RJ,Lall VK,Maxeiner S,Willecke K,Deuchars J,King AE

40-7000 was used in immunohistochemistry to identify Cx45-positive neurones in the adult spinal dorsal horn

Wed May 01 00:00:00 UTC 2013

Connexin and AMPA receptor expression changes over time in the rat olfactory bulb.

Neuroscience

Corthell JT,Fadool DA,Trombley PQ

40-7000 was used in western blot to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits.

Thu Oct 11 00:00:00 UTC 2012

Sex differences in expression and subcellular localization of heart rhythm determinant proteins.

Biochemical and biophysical research communications

Thomas NM,Jasmin JF,Lisanti MP,Iacobas DA

40-7000 was used in immunocytochemistry to investigate the effect of sex differences on cardiac gene and protein expression.

Fri Mar 04 00:00:00 UTC 2011

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.

Journal of cellular and molecular medicine

Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM

Fri May 01 00:00:00 UTC 2009

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.

Journal of cellular and molecular medicine

Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM

Fri May 01 00:00:00 UTC 2009

Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Li X,Kamasawa N,Ciolofan C,Olson CO,Lu S,Davidson KG,Yasumura T,Shigemoto R,Rash JE,Nagy JI

40-7000 was used in Western Blotting and Immunohistochemistry-Immunofluorescence to demonstrate that in Cx45-expressing neurons of IPL, Cx45 is almost always accompanied by Cx36, forming bihomotypic gap junctions.

Wed Sep 24 00:00:00 UTC 2008

The Journal of neuroscience : the official journal of the Society for Neuroscience

Li X,Kamasawa N,Ciolofan C,Olson CO,Lu S,Davidson KG,Yasumura T,Shigemoto R,Rash JE,Nagy JI

Wed Sep 24 00:00:00 UTC 2008

Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.

Journal of neurocytology

Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI

Thu Sep 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: connexin 45; Connexin-45; Cx45; Gap junction alpha-7 protein; Gap junction gamma-1 protein; gap junction membrane channel protein alpha 7

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Gene Aliases: C130009G16Rik; Cnx45; Cx45; Cxn-45; Gja-7; Gja7; Gjc1

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UniProt ID: (Mouse) P28229

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Entrez Gene ID: (Mouse) 14615

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Function(s)

ion channel activity gap junction channel activity protein binding

Process(es)

vasculogenesis cell communication cell-cell signaling chemical synaptic transmission heart development visual perception ion transmembrane transport cell development cardiac muscle tissue development transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Connexin 45 Polyclonal Antibody

Connexin 45 Antibody (40-7000) in ICC/IF

Product Details

40-7000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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