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Invitrogen
Connexin 45 Polyclonal Antibody
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Connexin 45 Antibody (PA5-99394) in IHC (P)
Immunohistochemistry analysis of Connexin 45 in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Connexin 45 polyclonal antibody (Product # PA5-99394) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99394
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GJC1(Accession P36383), corresponding to amino acid residues E341-K391.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818327
Product Specific Information
Antibody detects endogenous levels of total Connexin 45 / GJA7.
Target Information
Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: connexin 45; Connexin-45; CTC-296K1.4; Cx45; Gap junction alpha-7 protein; gap junction channel protein connexin 45; Gap junction gamma-1 protein; gap junction membrane channel protein alpha 7; gap junction protein, gamma 1, 45kDa
Gene Aliases: C130009G16Rik; Cnx45; CX45; Cxn-45; Gja-7; GJA7; GJC1
UniProt ID: (Human) P36383, (Rat) A4GG66, (Mouse) P28229
Entrez Gene ID: (Human) 10052, (Rat) 266706, (Mouse) 14615
vasculogenesis
transport
muscle contraction
cell-cell junction assembly
chemical synaptic transmission
visual perception
gap junction assembly
ion transmembrane transport
cell development
cardiac muscle tissue development
atrial cardiac muscle cell action potential
cell-cell signaling
heart development
cell communication
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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