Invitrogen

Connexin 50 Monoclonal Antibody (C6)

1 Published Figure

16 References

View all (7) Connexin 50 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

Connexin 50 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Connexin 50 Antibody (33-4300) in ICC/IF

Immunofluorescent analysis of Connexin 50 was done on 90% confluent log phase CaCo-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at... View More

Connexin 50 Antibody in Western Blot (WB)

Connexin 50 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

33-4300

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

Immunogen

This antibody was prepared against urea/alkali stripped sheep lens membranes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533120

Target Information

This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fabbiani G,Reali C,Valentín-Kahan A,Rehermann MI,Fagetti J,Falco MV,Russo RE

33-4300 was used in Immunohistochemistry to study the role of connexin signalling in the central canal in the reactivation of latent stem cell after spinal cord injury.

Wed Mar 11 00:00:00 UTC 2020

Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes.

American journal of physiology. Endocrinology and metabolism

Pelletier RM,Akpovi CD,Chen L,Vitale ML

33-4300 was used in Western Blotting to indicate that the db mutation-induced impairment of meiosis may arise from imbalances in cholesterol metabolism and upregulated Cx43 expression and phosphorylation in tubules.

Mon Jan 01 00:00:00 UTC 2018

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.

Cell and tissue research

Rodriguez-Jimenez FJ,Alastrue A,Stojkovic M,Erceg S,Moreno-Manzano V

33-4300 was used in immunocytochemistry and western blot to investigate modulation of Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells by connexin 50

Mon Aug 01 00:00:00 UTC 2016

Expression and Localization of Connexins in the Outer Retina of the Mouse.

Journal of molecular neuroscience : MN

Bolte P,Herrling R,Dorgau B,Schultz K,Feigenspan A,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunohistochemistry (frozen) to investigate the counterpart of connexin36 at gap junctions between rods and cones.

Mon Feb 01 00:00:00 UTC 2016

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells

Thu Oct 01 00:00:00 UTC 2015

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 UTC 2012

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 UTC 2012

PKCγ, role in lens differentiation and gap junction coupling.

Current eye research

Das S,Wang H,Molina SA,Martinez-Wittinghan FJ,Jena S,Bossmann LK,Miller KA,Mathias RT,Takemoto DJ

33-4300 was used in western blot to use knock out mice to determine the role of PKCgamma in the regulation of gap junction coupling in the normal lens.

Fri Jul 01 00:00:00 UTC 2011

Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas.

Annals of the New York Academy of Sciences

Willenberg HS,Schott M,Saeger W,Tries A,Scherbaum WA,Bornstein SR

33-4300 was used in immunohistochemistry - paraffin section to examine connexin expression in chromaffin cells of normal human adrenal glands and benign and malignant pheochromocytomas

Tue Aug 01 00:00:00 UTC 2006

Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia.

Visual neuroscience

Zahs KR,Ceelen PW

33-4300 was used in immunohistochemistry - frozen section to study connexins in mammalian Muller cells

Mon Jun 05 00:00:00 UTC 2006

Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels.

Investigative ophthalmology & visual science

Zampighi GA,Planells AM,Lin D,Takemoto D

Thu Sep 01 00:00:00 UTC 2005

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 UTC 2004

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 UTC 2004

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 UTC 2001

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: alpha 8 connexin; cell surface glycoprotein; connexin 50; Connexin-49; Connexin-50; Cx49; Cx50; gap junction alpha 8; Gap junction alpha-8 protein; gap junction membrane channel protein alpha 8; gap junction membrane channel protein alpha-8; gap junction protein alpha 8 50kDa; gap junction protein, alpha 8, 50kDa; Lens fiber protein MP70; lens intrinsic membrane protein MP70; lens opacity 10; MP38; MP64

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Gene Aliases: Aey5; CAE; CAE1; Cnx50; CTRCT1; CX50; CZP1; GJA8; Lop10; MP70

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UniProt ID: (Sheep) P55917, (Human) P48165, (Mouse) P28236

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Entrez Gene ID: (Sheep) 100170231, (Human) 2703, (Rat) 29601, (Mouse) 14616

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Function(s)

gap junction channel activity channel activity protein binding

Process(es)

lens development in camera-type eye cell-cell signaling camera-type eye development protein homooligomerization transmembrane transport transport visual perception cell communication

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Connexin 50 Monoclonal Antibody (C6)

Connexin 50 Antibody (33-4300) in ICC/IF

Product Details

33-4300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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