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Invitrogen
Creatine Kinase BB Monoclonal Antibody (CK-CYK/21E10)
Product Details
MA5-33339
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
CK-CYK/21E10
Immunogen
Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type (SNSHNALKLRFPAEDEF)
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815477
Product Specific Information
Product is supplied at 1 mL (100 µL/mL). Application notes: Western blot - Under reducing conditions, antibody detected a protein with a band size of 43-46 kDa. IHC-F- tissue was fixed for 2 hours in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1 M PB. The tissue was stored until use in 1 % paraformaldehyde. The tissue was blocked with 1% normal swine serum. IHC-P - antigen retrieval: 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C; endogenous peroxidise blocking by using 3 % H2O2 in PBS for 30 min; blocking with 1% normal swine serum for 30 min.; Purkinje cells serve as positive control and connective tissue as negative control.
Positive control: Brain lysate, Human Purknije cells; Negative control: Human fibroblasts
Target Information
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-CK; Brain creatine kinase; CPK-B; Creatine kinase B chain; Creatine kinase B-type; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; Creatine phosphokinase B-type; Creatine phosphokinase M-type; epididymis luminal protein 211; epididymis secretory protein Li 29
Gene Aliases: B-CK; BCK; Ck-3; Ck3; CKB; CKBB; Ckbr; HEL-211; HEL-S-29
UniProt ID: (Human) P12277, (Rabbit) P00567, (Rat) P07335, (Mouse) Q04447
Entrez Gene ID: (Human) 1152, (Rabbit) 100009085, (Rat) 24264, (Mouse) 12709
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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