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Invitrogen
Creatine Kinase MM Polyclonal Antibody
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Creatine Kinase MM Antibody (PA5-95546) in IHC (P)
Immunohistochemical analysis of Creatine Kinase MM in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Creatine Kinase MM antibody (Product # PA5-95546) overnight at 4°C. Biot... View More
Product Details
PA5-95546
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Creatine Kinase MM recombinant protein (Position: F3-K381).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807348
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human CNE whole cell, human COLO-320 whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue. IHC: rat cardiac muscle tissue, mouse cardiac muscle tissue, human mammary cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CPK-M; Creatine kinase M chain; Creatine kinase M-type; creatine kinase, muscle; Creatine phosphokinase M-type; M-CK
Gene Aliases: CKM; CKMM; M-CK; MCK
UniProt ID: (Human) P06732, (Rat) P00564, (Mouse) P07310
Entrez Gene ID: (Human) 1158, (Rat) 24265, (Mouse) 12715
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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