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Invitrogen
CrkL Polyclonal Antibody
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CrkL Antibody (PA5-28622) in ICC/IF
Immunofluorescence analysis of Crk-like protein was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CrkL Polyclonal Antibody (Product # PA5-28622) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
PA5-28622
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546098
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, U2OS.
Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (81%), Xenopus laevis (87%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The CRKL gene encodes the CRK-like protein, an adaptor protein that plays a crucial role in intracellular signaling pathways related to cell proliferation, differentiation, and migration. CRKL contains SH2 and SH3 domains that facilitate interactions with other signaling molecules, influencing multiple pathways such as the MAPK, PI3K, and STAT pathways. It is involved in processes like cytoskeleton reorganization and immune cell signaling, with significant implications for development and cancer. CRKL is notably associated with Philadelphia chromosome-positive leukemias, where it interacts with the BCR-ABL fusion protein, contributing to leukemogenesis. Additionally, dysregulation of CRKL can affect cardiovascular development, leading to congenital heart defects. Research into CRKL focuses on understanding its role in signal transduction and its potential as a therapeutic target due to its involvement in oncogenic and developmental pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Crk-like protein; crk-like protein {ECO:0000250|UniProtKB:P46109}; crkl {ECO:0000312|EMBL:AAH85865.1, ECO:0000312|RGD:1308531}; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog-like
Gene Aliases: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol
UniProt ID: (Human) P46109, (Mouse) P47941, (Rat) Q5U2U2
Entrez Gene ID: (Human) 1399, (Mouse) 12929, (Rat) 287942
blood vessel development
pattern specification process
heart development
organ morphogenesis
anterior/posterior pattern specification
thymus development
parathyroid gland development
biological_process
activation of MAPKK activity
JNK cascade
Ras protein signal transduction
positive regulation of cell proliferation
intracellular signal transduction
cell-cell adhesion
positive regulation of substrate adhesion-dependent cell spreading
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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