Proteintech

Cryptochrome 2 Polyclonal Antibody

Cryptochrome 2 Antibody in Immunocytochemistry (ICC/IF)

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Cryptochrome 2 Antibody (13997-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed SH-SY5Y cells using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cryptochrome 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cryptochrome 2 Antibody in Western Blot (WB)

Cryptochrome 2 Antibody in Immunoprecipitation (IP)

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Product Details

13997-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cryptochrome 2 Fusion Protein Ag5085 (243-593 aa encoded by BC041814)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: HLERKAWVA NYERPRMNAN SLLASPTGLS PYLRFGCLSC RLFYYRLWDL YKKVKRNSTP PLSLFGQLLW REFFYTAATN NPRFDRMEGN PICIQIPWDR NPEALAKWAE GKTGFPWIDA IMTQLRQEGW IHHLARHAVA CFLTRGDLWV SWESGVRVFD ELLLDADFSV NAGSWMWLSC SAFFQQFFHC YCPVGFGRRT DPSGDYIRRY LPKLKAFPSR YIYEPWNAPE SIQKAAKCII GVDYPRPIVN HAETSRLNIE RMKQIYQQLS RYRGLCLLAS VPSCVEDLSH PVAEPSSSQA GSMSSAGPRP LPSGPASPKR KLEAAEEPPG EELSKRARVA ELPTPELPSK DA (243-593 aa encoded by BC041814)

Target Information

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic Activation of Circadian Clock Genes Elicits Inflammation in Experimental Murine Dry Eye.

Ocular immunology and inflammation

Wei R,Chen Q,Zheng Q,Reinach PS,Tan X,Pan C,Xu W,Tong L,Chen W

Sun Sep 01 00:00:00 UTC 2024

7,8-Dihydroxyflavone alleviates cardiac fibrosis by restoring circadian signals via downregulating Bmal1/Akt pathway.

European journal of pharmacology

Hang PZ,Liu J,Wang JP,Li FF,Li PF,Kong QN,Shi J,Ji HY,Du ZM,Zhao J

Thu Jan 05 00:00:00 UTC 2023

Role of Bmal1 in Type 2 Diabetes Mellitus-Related Glycolipid Metabolic Disorder and Neuropsychiatric Injury: Involved in the Regulation of Synaptic Plasticity and Circadian Rhythms.

Molecular neurobiology

Gao X,Wei Y,Sun H,Hao S,Ma M,Sun H,Zang D,Qi C,Ge J

Tue Aug 01 00:00:00 UTC 2023

Cryptochrome 2 acetylation attenuates its antiproliferative effect in breast cancer.

Cell death & disease

Xia K,Li S,Yang Y,Shi X,Zhao B,Lv L,Xin Z,Kang J,Ren P,Wu H

Thu Apr 06 00:00:00 UTC 2023

Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium.

Anesthesia and analgesia

Dulko E,Jedrusiak M,Osuru HP,Atluri N,Illendula M,Davis EM,Beenhakker MP,Lunardi N

Sat Jul 01 00:00:00 UTC 2023

Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast through the Regulation of Wnt/Β-Catenin Signaling Pathway.

BioMed research international

Ma C,Yang C,Xie T,Dai W,Ma J

Wed Sep 07 00:00:00 UTC 2022

The association between disruption of the circadian rhythm and aggravation of colitis in mice.

Gastroenterology report

Chen YD,Zhao RF,Zheng G,Ling FM,Li JR,Xu MY,Guo D,Zhang QL,Li S,Zhu LR

Sat Jul 16 00:00:00 UTC 2022

Melatonin pretreatment prevents propofol-induced sleep disturbance by modulating circadian rhythm in rats.

Experimental neurology

Yin XL,Li JC,Xue R,Li S,Zhang Y,Dong HJ,Li Y,Wang HL,Zhang YZ

Mon Aug 01 00:00:00 UTC 2022

METTL3 Regulates Liver Homeostasis, Hepatocyte Ploidy, and Circadian Rhythm-Controlled Gene Expression in Mice.

The American journal of pathology

Barajas JM,Lin CH,Sun HL,Alencastro F,Zhu AC,Aljuhani M,Navari L,Yilmaz SA,Yu L,Corps K,He C,Duncan AW,Ghoshal K

Sat Jan 01 00:00:00 UTC 2022

Age-dependent effects of a high-fat diet combined with dietary advanced glycation end products on cognitive function and protection with voluntary exercise.

Food & function

Luo L,Li R,Wang G,Chen J,Chen L,Qin LQ,Yu Z,Wan Z

Wed Apr 20 00:00:00 UTC 2022

Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.

Journal of agricultural and food chemistry

Li R,Xiao J,Cao Y,Huang Q,Ho CT,Lu M

Wed Jan 26 00:00:00 UTC 2022

Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.

Science advances

Lee Y,Fong SY,Shon J,Zhang SL,Brooks R,Lahens NF,Chen D,Dang CV,Field JM,Sehgal A

Mon Feb 01 00:00:00 UTC 2021

CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression.

Cell death and differentiation

Zhang D,Ni N,Wang Y,Tang Z,Gao H,Ju Y,Sun N,He X,Gu P,Fan X

Fri Jan 01 00:00:00 UTC 2021

hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.

Cell death & disease

Wan X,Zhu L,Zhao L,Peng L,Xiong J,Yang W,Yuan J,Liang F,Zhang K,Chen K

Fri Mar 19 00:00:00 UTC 2021

Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.

Journal of immunology research

Tan X,Cao F,Tang F,Lu C,Yu Q,Feng S,Yang Z,Chen S,He X,He J,Weng L,Sun L

Mon Dec 20 00:00:00 UTC 2021

Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.

Molecular therapy. Nucleic acids

Tang Z,Xu T,Li Y,Fei W,Yang G,Hong Y

Fri Mar 06 00:00:00 UTC 2020

Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.

Molecular therapy. Nucleic acids

Tang Z,Xu T,Li Y,Fei W,Yang G,Hong Y

Fri Mar 06 00:00:00 UTC 2020

Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.

Molecular therapy. Nucleic acids

Tang Z,Xu T,Li Y,Fei W,Yang G,Hong Y

Fri Mar 06 00:00:00 UTC 2020

Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.

Molecular therapy. Nucleic acids

Tang Z,Xu T,Li Y,Fei W,Yang G,Hong Y

Fri Mar 06 00:00:00 UTC 2020

G1/S cell cycle regulators mediate effects of circadian dysregulation on tumor growth and provide targets for timed anticancer treatment.

PLoS biology

Lee Y,Lahens NF,Zhang S,Bedont J,Field JM,Sehgal A

Mon Apr 01 00:00:00 UTC 2019

Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.

Free radical biology & medicine

Ji G,Lv K,Chen H,Wang Y,Zhang Y,Li Y,Qu L

Sun Dec 01 00:00:00 UTC 2019

Cryptochromes-Mediated Inhibition of the CRL4^Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Current biology : CB

Rizzini L,Levine DC,Perelis M,Bass J,Peek CB,Pagano M

Mon Jun 17 00:00:00 UTC 2019

The NRON complex controls circadian clock function through regulated PER and CRY nuclear translocation.

Scientific reports

Lee Y,Shen Y,Francey LJ,Ramanathan C,Sehgal A,Liu AC,Hogenesch JB

Thu Aug 15 00:00:00 UTC 2019

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats.

Current medical science

Huang WY,Zou X,Lu FE,Su H,Zhang C,Ren YL,Fang K,Xu LJ,Wang KF,Chen QJ,Dong H

Wed Aug 01 00:00:00 UTC 2018

Effect of Jiaotai Pill () on intestinal damage in partially sleep deprived rats.

Chinese journal of integrative medicine

Huang WY,Zou X,Lu FE,Zhang C,Ren YL,Xu LJ,Wang KF,Dong H

Fri Dec 01 00:00:00 UTC 2017

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

eLife

Lee Y,Jang AR,Francey LJ,Sehgal A,Hogenesch JB

Sat Aug 29 00:00:00 UTC 2015

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

eLife

Lee Y,Jang AR,Francey LJ,Sehgal A,Hogenesch JB

Sat Aug 29 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: cryptochrome 2 (photolyase-like); Cryptochrome-2; growth-inhibiting protein 37

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Gene Aliases: AV006279; CRY2; D130054K12Rik; HCRY2; KIAA0658; PHLL2

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UniProt ID: (Human) Q49AN0, (Mouse) Q9R194

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Entrez Gene ID: (Human) 1408, (Rat) 170917, (Mouse) 12953

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Function(s)

transcription regulatory region sequence-specific DNA binding transcription factor activity, transcription factor binding DNA binding damaged DNA binding single-stranded DNA binding deoxyribodipyrimidine photo-lyase activity DNA (6-4) photolyase activity protein binding blue light photoreceptor activity phosphatase binding ubiquitin binding FAD binding DNA photolyase activity photoreceptor activity kinase binding protein kinase binding nuclear hormone receptor binding nucleotide binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter photoreactive repair transcription, DNA-templated circadian rhythm blue light signaling pathway protein-chromophore linkage negative regulation of phosphoprotein phosphatase activity circadian regulation of gene expression glucose homeostasis regulation of circadian rhythm negative regulation of circadian rhythm entrainment of circadian clock by photoperiod negative regulation of transcription, DNA-templated regulation of sodium-dependent phosphate transport negative regulation of glucocorticoid receptor signaling pathway DNA repair protein import into nucleus signal transduction lipid storage response to insulin negative regulation of glucocorticoid secretion regulation of transcription, DNA-templated rhythmic process response to stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cryptochrome 2 Polyclonal Antibody

Cryptochrome 2 Antibody (13997-1-AP) in ICC/IF

Product Details

13997-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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